弓形虫生活史.docx

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SeroprevalenceofToxoplasmagondiiInfectioninStrayandHousehold
CatsinGuangzhou,China
,,,,,,

LaboratoryofParasitology,CollegeofVeterinaryMedicine,SouthChinaAgriculturalUniversity,483WushanStreet,TianheDistrict,Guangzhou,GuangdongProvince510642,People'sRepublicofChina
CenterforParasiticOrganisms,StateKeyLaboratoryofBiocontrol,SchoolofLifeSciences,SunYat-SenUniversity,Guangzhou,GuangdongProvince510275,People'sRepublicofChina
DepartmentofParasitology,SchoolofPublicHealthandTropicalMedicine,SouthernMedicalUniversity,Guangzhou,GuangdongProvince510515,People'sRepublicofChina
RunningHead:ToxoplasmagondiiinfectionincatsinGuangzhou,China
Correspondence:
,SouthChinaAgriculturalUniversity,483WushanStreet,TianheDistrict,Guangzhou,GuangdongProvince510642,People':+862085283730;Fax:+862085283730;
E-mail:******@
Impacts
ThisarticleshowsnewfiguresonthepresentsituationofToxoplasmagondiiinfectioninstrayandhouseholdcatsinmetropolitanGuangzhou,China.
,whichalsoindicatesthezoonoticsignificanceoftheprevalence.
Thedatapresentedprovide“base-line”,China.
Summary
Theprevalenceofanti-ToxoplasmagondiispecificIgGinstrayandhouseholdcatsinGuangzhou,ChinawasdeterminedbyELISAonserumsamplesfrom206cats(117straysand89households),%.Theinfectionrateinstraycats(%)wassignificantlyhigher(p<)thaninhouseholdcats(%).Therateofinfectionbetweenmaleandfemalecatsofbothgroupswasnotsignificantlydifferent(p>
),%%formaleandfemaleinstraycats,respectively,and18%%,especiallyinstraycats,,.
Keywords:
Cat;ELISA;Guangzhou;Seroepidemiology;Toxoplasmagondii;Toxoplasmosis
Introduction
Toxoplasmagondiiisanobligateintracellularprotozoaparasitethatcaninfectavarietyofcelltypesfromawiderangeofmammalsandbirdsthroughouttheworld,includinghumansandnonhumanprimates(Jacobsetal.,1998).,theprimaryinfectionduringpregnancyinwomenandanimalsmaycauseabortion,fetalabnormalitiesorprenataldeath(Cooketal.,2000).Moreimportantly,(MontoyaandLiesenfeld,2004).
Felidsplayacrucialroleintheepidemiologyofthisparasiticdisease,becausetheyaretheonlydefinitivehost,sheddingandexcretingmillionsofinfectiveoocystsinashortperiodoftime(Dubey,1994),.
(;Yuetal.,2006;;unfortunately,thesereportswerepublishedonlyintheChineselanguage).However,,,China.
Materialandmethods
Animals
Biometricdataforcatsincludingage,.
Bloodsamplingandserumpreparation
Bloodsamples()-20°CuntilneededforELISA.

(Shenzhen,China)accordingtothemanufacturer',-(1:100)inthetestwellandsubsequentwashing,,(OD)at450nmwasreadusingaphotometer(BIO-RAD).
Arelativeratepercent(IRPC)valuewasobtainedusingthefollowingformula:
IRPC— OD450(sample)-meanOD450(blankcontrol)
meanOD450(negativecontrol)-meanOD450(blankcontrol)
<,andpositiveifIRPC>(meanOD450negativecontrol-meanOD450blankcontrol).
Statisticalanalysis
Differencesintheseroprevalenceofinfectedcatsbetweenstrayandhouseholdgroups,betweenmaleandfemalecatswereanalyzedusingChiSquareTestinSPSSforWindows,(,SPSSInc.).ThecorrelationbetweentheratesofinfectionindifferentagegroupswascalculatedwithExcel(Microsoft®Excel2003).
Resultsanddiscussion
Inthisinvestigation,theELISAmethodwasutilizedduetotheeaseofitsapplication,anditshighsensitivityandspecificitywhencomparedwithothertestmethods(LappinandPowell,1991).Inthisstudy,206cats(,82weremale,85werefemaleand39unknownsex(Table1).AsshowninTable1,%.%%inhouseholdcats(Table1),%%indifferentagegroups(Table2).
,%,whichwaslowerthanthatreportedinothercountries(Miroetal.,2004;Haddadzadehetal.,2006),andinShanghaiandHebeiprovinceinChina(;),butsimilartothatreportedinBeijing(Yuetal.,2006).
Inthepresentinvestigation,,theseroprevalenceofinfectioninstraycats(%)wassignificantlyhigher(p<)thanthatofhouseholdcats(%).ThisiscomparabletotheresultsofHaddadzadehetal.(2006),whichshowedasignificantdifferencebetweenstrays(aseroprevalenceof90%)andhouseholdcats(36%)inTehran,.(2003)alsofoundsignificantdifferences,%instraycats,%inhouseholdcatsinBarcelona,,andtheirdietincludswildbirds,rodents,andToxoplasma-infectedplacentasandstillbornfetuses(Frenkel
1990;).However,studiesdoneintheUnitedStatesdidnotshowsignificantdifferencesbetweenhouseholdcatsandstraycats(Hilletal.,2000;DeFeoetal.,2002).
,SumnerandAckland(1999)tested103serumsamplesfromdomesticcatsinMelbourne,.(2006)(Table1),%(18of82),%(22of85),butthedifferencewasnotsignificant(p〉)(Table1).However,therewasasignificantlyhigherseroprevalenceinmalestraysthaninfemalesreportedbyMiroetal.(2004).
Amongstcatswhoseageswereknown,asignificantlyhigher(%)prevalenceofinfectionwasdetectedin>%incats<,seroprevalencevariesindifferentagegroups,%%.Thedifferenceinprevalencefoundwithrespecttoage(adultsversusjuveniles)coincideswithotherstudieswherehigherprevalenceisobservedintheadultgroupthaninthejuvenileone(Smithetal.,1992;Gaussetal.,2003),(Frenkel,1990;Hilletal.,2000).
Conclusions
,especiallyinstraycats,
,.
Acknowledgements
Thisworkissupported,inpart,bygrantsfromtheNationalBasicResearchProgramof
China(),theNationalSpecialResearchProgramsforNon-ProfitTrades(Agriculture)(),theKeyResearchProgramsinNaturalSciencesforInstitutionsofHigherEducationinGuangdongProvince(),andtheProgramforChangjiangScholarsandInnovativeResearchTeaminUniversity().
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