一年级语文上册《四季》课件.ppt

究通过对200尾青海湖裸鲤个体的GH基因部分片段进行直接测序,进而分析比
较,并与青海湖裸鲤生长性状之间进行关联分析,初步筛查得到了3个与生长性状相关的SNP位点。对于位点SNP68,的个体在体高和体重上比基因型CT具有优势;对于位点SNP80,基因型AA的个体在体长、体重、体高、臀鳍基长比基因型AT的个体均具有生长优势;对于位点SNP148,基因型AG 个体在体长、体重、体高、臀鳍基长比基因型GG的个体具有生长优势。该研究结果为开展分子标记辅助育种奠定基础。
关键词:青海湖裸鲤 AFLP 形态性状体质量 SNP
Abstract
Gymnocypris przewalskii, the naked carp is one of the endangered species in China. The selective breeding of the parent naked carp is the most important work in protection and restoration of wild naked carp. In this thesis, first, the relationships between body weight and related morphological characters were determined. Then, the naked carp groups in different tributaries were analysied by the means of amplified fragment length polymorphism (AFLP) finger printing. Finally, SNP locis were determinded by sequence alignment method, combining with the SNPs locis and morphological characters, three SNPS loci associated with growth traits were screened. Specific contents and results are as follows:
In this study, to determine the relationship between body weight and morphological traits, a total amount of 200 2-years-old individuals were sampled for measuring the body wheight and nine metric traits, including the total length, standard length, head length, body height, interorbital width, length before anus, body width, anal fin base. The relationship between morphological characters and body weight was quantified by correlation analysis, multiple regression and path analysis methods. It was showed the correlation coefficient between most morphometric traits
and body weight reached extremely significant level (p<). Path analysis showed
that the main factor in direct impact on body weight is standard length, followed by body heght and long anal fin base. The morphometric attributes standard length(X2), body height(X5) and anal fin base(X9) were used to establish the multiple regression equations as Y= X2+ X5+ X9-. According to the regression coefficient test, the influence of