塔里木大学2013届应用化学专业毕业生毕业论文.docx

目录
摘要 1
关键词 1
0 引言 2
1 实验仪器和方法 4
仪器 4
试剂 4
4
方法 4
昆仑雪菊的黄***含量测定 4
黄***的抗氧化测定 5
清除超氧阴离子自由基能力的测定 5
***溶液清除羟自由基能力测定 5
对DPPH自由基清除活性的测定 6
抗脂质氧化能力测定 6
2 实验结果与讨论 6
***提取和含量测定 6
6
样品测定结果 7
***的还原性测定结果 7
***的抗氧化测定结果 8
8
9
10
抗脂质氧化能力实验结果 12
3 结论 12
参考文献 13
致谢 14
昆仑雪菊总黄***抗氧化活性研究
摘要【目的】采用超声萃取法用四种不同的溶剂提取昆仑雪菊中总黄***类化合物,测定其含量并研究其抗氧化性。【方法】利用芦丁标准曲线对黄***的含量进行测定,采用DPPH法,结晶紫分光光度法,邻***自氧化法,抗脂质法研究总黄***的抗氧化能力,用普鲁士蓝法对其还原能力进行研究。【结果】70%乙醇为溶剂提取率最高,石油醚为溶剂提取率最低。雪菊总黄***有清除超氧负离子自由基,羟基自由基,DPPH 自由基的能力,以及较强的抗氧化活性。【结论】在试验浓度范围内,%,%,%,并且抗氧化能力与总黄***的含量相关。黄***作为天然的抗氧化剂,具有广阔的应用前景。
关键词昆仑雪菊;总黄***;还原性;抗氧化性
Studying on the Flavonoids in Kunlun Chrysanthemum of Antioxidant Activity
Abstract:【Objective】 Explore the optimal experimental condition for the measure of the total antioxidant capacity of natural product based on Ce(IV) reducing capacity by optimizing the determining of antioxidant capacity of chlorogenic acid via Ce(IV).【Methods】The fluorescent Ce(IV) exhibited strong fluorescence at excitation wavelength of 319nm. The method is based on the electron-transfer (ET) reaction between Ce(IV) ion and chlorogenic acid and subsequent determination of reducing of Ce(IV) ions by a fluorometric method. We use the single factor experiment method to optimize experiment conditions from the solvent, temperature, reaction time, PH value and the mole ratio. 【Conclusion】 Experiments show that mol/L of sulfuric acid is the best sulfate solvents, temperature is at room temperature, reaction time at 60 s can be a standard, PH value is between 0 and 1,and the molar ratio of is in :1. The new measure of total antioxidant capacity of natural product broad application prospect. The new measure of total antioxidant capacity of natural product has broad application prospect.
Keywords: Kunlun s