中南大学硕士学位论文 中文摘要 摘 要 目的:通过检测骨桥蛋白(OPN)、小凹蛋白()在骨关节炎不同退变程度关节软骨中的表达,(OA)发生与发展过程中的作用。 方法:选取10例正常关节软骨标本和26例骨关节炎软骨标本, 依据Outerbridge标准从上述软骨标本中凿取正常和OA不同退变程度软骨块共50例,随后根据关节软骨改良Mankin病理评分法将上述软骨块进行分组:正常关节软骨标本9例(A组)、0A软骨轻度退变标本10例(B组)、0A软骨中度退变标本15例(C组)、0A软骨重度退变标本13例(D组)(其中有3例标本因脱片或取材失误未纳入后续实验)。运用免疫组化方法(SABC法)检测上述四组关节软骨中OPN ,。 结果:,其表达的平均灰度值分别为(A组)±、(B组)+8。02、(C组) 、(D组)+。各组之间OPN的表达比较均具 有显著性差异(p<)。 ,其表达的平均灰度值分别为(A组)+、(B组)-4-、(C组) +、(D组)+。、 C、D组,B组与C组,B组与D组两两比较均有显著性差异(P<)。 (P>)。 (r=,P<)即OPN的表达与关节软骨退变程度呈正相关。 中南大学硕士学位论文中文摘要 关(,P<)。(F ,P<)。 结论:。 ,其表达随软骨的退变程度增加而显著降低, 。 。 关键词:骨关节炎(0A),OPN,,Mankin分级, 免疫组化 Ⅱ 中南大学硕士学位论文 英文摘要 ABSTRACT Objective This study is undertaken to approach the correlations between OPN and Caveolin一1 in pathogenesis and progression of osteoarthritis(OA)by detecting the expression of OPN and Methods To collect 36 cartilage posed of OA(26) and normal joints(1 0).50 cartilage tissues with the form of cm X cm X were cut from these specimens according to the Outerbridge the cartilage tissues included normal and impaired cartilage all the samples were ascribed to four groups of normal(A)、minor(B)、moderate(C)and severe(D)on the basis of the improved grading system of expression of OPN and Caveolin·1 were detected in these samples by the immunohistochemistry(SABC)and the expression result statistics were analysed by SPSS 1 . Results were detected on the