c反应蛋白对内皮细胞株ecv304细胞释放前列环素、纤溶酶原激活物抑制剂的影响-心血管内科专业毕业论文.docx

中文躺婺
24,{、瓣与i2小辩穗跑较￡,尹一O,042);CRP(50mg/L)魑ECV304缨腿PAI-I静分泌箨辩闽点蚜商予对照缀(3小时与对照组稠眈￡一3。37,尸= 0。028;6小辩海瓣照鳃勰拢#,;12小时与对照鳃稳魄。一
13。86,P一0,000;24与对照熄棚比小时￡一8+oo,P—o,00t)。(2)CRP缀鞍对照缀(CRPOmg/L)明爨减少ECV304缨腿分泌PGl2(尸一0+000),并髓CRP浓度增翔,PGh分泌减少;CRP(50mg/L)缀ECV304缁簸PGh游分泌12小澍两髓辩阉惩长瑟璺递增趋势(6小时与3小鞲寸旗魄较t一17。41,P—;12
小砖与6,j、瓣秘跣较s—,P=),24小游缀臻爨魄12小时缀溅少(}一3+23,P—);CRP(50mg/L)缝ECV304细胞PGl2的分泌3小时大于对照缀≤o=3。86,P—O+018),6小瓣与对照缀秃菱黪(#一2。t3,∞, 12及24小时秘疆少予对照鳃({2及24小时￡一10。87、13。ll,P—O。000)。缕谂CRP可键遴癍液姻骢释放PAI-1,鳓裁PGIa分泌,霹熊躞搬管辩张发抗凝{乍糟缀蠲,稳馊懿镑痰挛、斑块瀚破裂、照竣影成,鬻嚣霹怒在熊致
ACS煞发生蔑麟中起鼙溪体爝。
关键遥炎瘸劫欺粥群疆钱惫性避躲练含征e菠斑簧向前列躲环豢纤溶麟原激活物籀铡裁~1
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硕士学位论文
The effects of CRP on the expression of PGh、PAl一1 by ECV304 cells
Name:Lai Xiao HUi Supervisor:Zhmag Qing Hua
ABSTRACT
Objective:To observe the possible effects of C·reactive protein汜RP)on the expression of prostaglandin 12(PGl2)、Plasminogen Activator Inhibitor-I(PAI—1) by ECV304 cells mad assess the possible role in AS and acute coronary syndromes(Acs)+
Medthods:Thr01]lgh incubation of ECV304 cells with different concentration ofCRP<O mg/L、25 mg/L、50mg/L、100mg/L)for 24 hours,to observe the dose effect of CRP in ECV304 cells+Through incubation of ECV304 cells with CRP (50medL)fordifferenttime(3hours、6hours、12hours、24 hours),toobserve the time effect of CRP itl ECV304 cells. measure the concentration of
6-keto*PGFIa、PAt-1 in cultural fluid by enzyme-linked immunosorbent assay (ELLISA)。Group datas are meana:SD,through SPSSl{.0 software,datas are analyed by one—way ANOVA or f-test。Differences were considered significant at a value of p<O。05,
Results:(1)Incubation ofECV304 cellswithCRP resultsin atime-(6hVS 3h t一4。84,P=;t2h VS 6h t=10+73,P—O,000:24h VS 12h≠=,P
∞O。042)and dose-dependent沪一O。ooo)increase in secreted PAI—l;at 3 hours PAI-1 exceed control group(3h t;3。37+P=;6h f ,P={ 12h t一13:86,P=O,000;24h t-=,)。(2)Incubation ofEeV3躺 cells with CRP results in dose—dependentrP=)decrease in secrete