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Molecular Biology Course
Section K: Transcription in prokaryotes
DNA
RNA
PROTEIN
today’s focus
K1 转录基本的原则
K2 RNA聚合酶
K3 The E. coli s70启动子
K4转录过程
综述, RNA合成的过程(起始,延伸,终止)
功能, a 亚基, b 亚基, b’亚基, sigma (s) 因子
启动子, s70大小, -10 序列, -35 序列,转录起始位点, 启动子效率
启动子结合,解旋, RNA 链起始,延伸, 终止(r蛋白因子)
Section K: Transcription in prokaryotes
Molecular Biology Course
K1:转录基本的原则
Transcription: 综述(相对复制而言)
The process of RNA synthesis: initiation, elongation, termination
Molecular Biology Course
K1-1: Transcription: an overview
Key terms defined in this section
(Gene VII)
Coding strand of DNA has the same sequence as mRNA. Downstream identifies sequences proceeding farther in the direction of expression; for example, the coding region is downstream of the initiation codon.
Gene X
Primary transcript
m7Gppp
AAAAAn
+1
upstream
downstream
Upstream identifies sequences proceeding in the opposite direction from expression; for example, the bacterial promoter is upstream from the transcription unit, the initiation codon is upstream of the coding region.
Transcription unit is the distance between sites of initiation and termination by RNA polymerase; may include more than one gene.
Promoter is a region of DNA involved in binding of RNA polymerase to initiate transcription
RNA Terminator is a sequence of DNA, represented at the end of the transcript, that causes RNA polymerase to terminate transcription.
RNA polymerases are enzymes that synthesize RNA using a DNA template (formally described as DNA-dependent RNA polymerases).
Primary transcript is the original unmodified RNA product corresponding to a transcription unit.
Replication: synthesis of two DNA strands using both parental DNA strands as templates. Duplication of a DNA molecule.
1 DNA molecule 2 DNA molecules
Transcription: synthesis of one RNA molecule using one of the two DNA strands as a template.
K1: Basic principles of transcription
comparison with replication
Synthesis of the Okazaki fragments
Section K: Transcription in prokaryotes
DNA
RNA
PROTEIN
today’s focus
K1 转录基本的原则
K2 RNA聚合酶
K3 The E. coli s70启动子
K4转录过程
综述, RNA合成的过程(起始,延伸,终止)
功能, a 亚基, b 亚基, b’亚基, sigma (s) 因子
启动子, s70大小, -10 序列, -35 序列,转录起始位点, 启动子效率
启动子结合,解旋, RNA 链起始,延伸, 终止(r蛋白因子)
Section K: Transcription in prokaryotes
Molecular Biology Course
K1:转录基本的原则
Transcription: 综述(相对复制而言)
The process of RNA synthesis: initiation, elongation, termination
Molecular Biology Course
K1-1: Transcription: an overview
Key terms defined in this section
(Gene VII)
Coding strand of DNA has the same sequence as mRNA. Downstream identifies sequences proceeding farther in the direction of expression; for example, the coding region is downstream of the initiation codon.
Gene X
Primary transcript
m7Gppp
AAAAAn
+1
upstream
downstream
Upstream identifies sequences proceeding in the opposite direction from expression; for example, the bacterial promoter is upstream from the transcription unit, the initiation codon is upstream of the coding region.
Transcription unit is the distance between sites of initiation and termination by RNA polymerase; may include more than one gene.
Promoter is a region of DNA involved in binding of RNA polymerase to initiate transcription
RNA Terminator is a sequence of DNA, represented at the end of the transcript, that causes RNA polymerase to terminate transcription.
RNA polymerases are enzymes that synthesize RNA using a DNA template (formally described as DNA-dependent RNA polymerases).
Primary transcript is the original unmodified RNA product corresponding to a transcription unit.
Replication: synthesis of two DNA strands using both parental DNA strands as templates. Duplication of a DNA molecule.
1 DNA molecule 2 DNA molecules
Transcription: synthesis of one RNA molecule using one of the two DNA strands as a template.
K1: Basic principles of transcription
comparison with replication
Synthesis of the Okazaki fragments
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