第七章 微生物生长繁殖与遗传变异
第一节微生物生长的测定 Measurement of cell growth
Total cell count (direct observation under microscope)
Viable count (plate count or colony count)
Spread plate method
Pour plate method
The plates required to have between 30 and 300 colonies.
一、直接计数法:总菌数法(死菌+活菌)
:
Measuring cell density:
Turbidity vs Cell density
液
二、间接计数法:活菌法
:广泛用于各种样品的检测
方法:(1)涂布法(菌长在表面)
(2)混菌法(菌长在表面和基内)优点:
缺点:
Viable count: Spread plate methodand Pour plate method
Three basic steps:
Dilution,
Plating
Incubation
Viable Count: Dilution
Viable Count: Plating
Incubation (and counting)
Spread plate method
Pour plate method
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