MicroRNA let-7i转染诱导的CD86-DCs对Treg作用的研究.doc



MicroRNA let-7i 转染诱导的 CD86-DCs 对
Treg 作用的研究#
吴健1,2,张毛毛1,2,刘芳1,2,韩晓丽1,2,谭妙欣1,2*
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(1. 哈尔滨医科大学,心肌缺血机理与诊疗技术教育部共建重点实验室,哈尔滨 150081;
2. 哈尔滨医科大学附属第二医院心血管内科,哈尔滨 150081)
摘要:目的进一步研究 miRNAlet-7i 的免疫调节作用是通过 DC 的何种亚群发挥作用的,以
及此亚群对 Treg 的影响。方法用免疫磁珠将 miRNAlet-7i 抑制剂处理的 DCs 分成两组:
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对 Treg 数量的影响,对上清液 IL-10 分泌的影响;Western blotting 检测 miRNAlet-7i 的
靶点 SOCS1 在不同细胞亚群的表达。结果转染 miRNAlet-7i 抑制剂明显降低了 LPS 刺激的
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诱导 T 细胞的低反应性和抗炎症细胞因子的分泌,并且抑制促炎症细胞因子的分泌,从
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关键词:树突状细胞;miRNAlet-7i;调节性 T 细胞;细胞因子
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The role of miRNA let-7i transfection-induced CD86-DC on
Treg action
WU Jian1,2, Zhang Maomao1,2, Liu Fang1,2, Han Xiaoli1,2, Tan Miaoxin1,2
(1. Key Laboratories of Education Ministry for Myocardial Ischemia Mechanismand Treatment,
Harbin Medical University, Harbin 150081;
2. Department of Cardiology, Second Affiliated Hospital of Harbin Medical University,
Harbin 150081)
Abstract: Objective Too further research through which DC subsets did miRNAlet-7i play a role
in immune regulation and the impact of this sub-group on Treg. Methods miRNAlet-7i inhibitor
transfected DC were divided into CD86+DCs and CD86-DCs using ic beads,
cocultured with T cells and observed the effect of CD86+DC and CD86-DC on T cell
proliferation、the number of Treg and IL-10 secretion. The expression of SOCS1 of different cell
subsets which is the target of miRNAlet-7i is detected by Western blotting. Results Transfecting
with miRNAlet-7i inhibitor significantly impeded DC surface expression of CD80 and CD86 and
the secretion of pro-inflammatory cytokine. There were two subpopulations of LPS-stimulated
DCs with downregulated miRNAlet-7i, CD86+DC and CD86-DC, and it was the CD86-DCs that
were more effective in inducing T cell hyporesponsiveness and the secretion of anti-inflammatory
cytokine,and inhibiting secretion of pro-inflammatory +CD25+Tregs, isolated from
CD86-DCs and T cells cocultured medium, can inhibition o