慢性间歇低氧对幼鼠海马神经细胞凋亡与caspase-12表达的影响.doc

慢性间歇低氧对幼鼠海马神经细胞凋亡及caspase-12表达的影响
作者:高原1,李源1,3,蔡晓红1,周永海2,张存雪1,4,李宁1,5 作者单位:(温州医学院附属育英儿童医院,浙江温州 325027,;;,河南郑州 450000;,山东济宁 272111;,广东深圳 518000)
【摘要】目的:探讨慢性间歇低氧对幼鼠海马神经细胞凋亡及caspase-12表达的影响。方法:取体重为80~100 g健康雄性SPF级SD幼鼠(3~4周龄)40只,随机分为间歇低氧2周(2IH)组、4周(4IH)组,空气对照2周(2C)组、4周(4C)组,每组10只。以间歇低氧舱建立慢性间歇低氧幼鼠模型。采用TUNEL法检测海马神经细胞凋亡,RT-PCR检测海马区caspase-12 mRNA表达,免疫组织化学染色检测海马区caspase-12蛋白的表达。结果:间歇低氧组凋亡指数(AI)、caspase-12 mRNA及其蛋白表达均高于对照组(均P <);4IH的AI,caspase-12 mRNA及其蛋白表达均高于2IH(均P <)。结论:慢性间歇低氧幼鼠海马神经细胞caspase-12 mRNA及其蛋白表达随间歇低氧时间延长而增加,内质网应激参与慢性间歇低氧幼鼠海马神经细胞凋亡过程。
【关键词】慢性间歇低氧;内质网应激;海马;凋亡;caspase-12
Effect of chronic intermittent hypoxia on apoptosis of rat's hippocampal cells and the expression of caspase-12 in immature rats GAO Yuan*, LI Yuan, CAI Xiao-hong, ZHOU Yong-hai, ZHANG Cun-xue, LI Ning. *Department of Pulmonology,Yuying Children's Hospital Affiliated to Wenzhou Medical College, Wenzhou, 325027
Abstract: Objective: To explore the effect of chronic intermittent hypoxia on apoptosis of hippocampal cells and the expression of caspase-12 in immature rat's. Methods: Forty male Sprague- Dawley rats (3~4 week,80~100 g) were randomly divided into four groups: 2-week intermittent hypoxia group (2IH),4-week intermittent hypoxia group (4IH),2-week control group with room air(2C) and 4-week control group with room air (4C), and ten rats in eac