中药联合TTT对视网膜神经保护作用的实验研究.doc

中药联合TTT对视网膜神经保护作用的实验研究
【摘要】目的:观察归芍地黄汤联合经瞳孔温热疗法(TTT)对兔视网膜神经的保护作用。方法:30只成年青紫蓝兔随机分为正常对照组、模型组、TTT治疗组、中药治疗组、中药联合TTT治疗组5组,每组6只。V)模型。造模4 w 后,中药治疗组、中药联合TTT治疗组予归芍地黄汤灌胃, g/(kg·d),每日1次,持续至造模后8 w;将TTT治疗组、中药联合TTT治疗组动物置于固定箱中,%美多丽-P滴眼液双眼散瞳,%倍诺喜行眼表麻醉后,在全视网膜镜下行TTT治疗。8 w后取眼球壁组织行HE染色,轴突用丽春红-亮绿染色法。结果:造模8 V;轴突染色结果显示中药联合TTT治疗组轴突染色面积和积分光密度值高于TTT治疗组和中药治疗组。结论:归芍地黄汤联合TTT对年龄相关性黄斑变性有更好的治疗效果。
【关键词】年龄相关性黄斑变性;脉络膜新生血管;经瞳孔温热疗法;神经保护作用
Abstract Objective:To observe the protective effect of Guishao Dihuang bined with transpupillary thermotherapy(TTT) on retina nerve of :30 rabbits were randomly divided into five groups(n=6):normal control group,model group, TTT treatment group,TCM treatment group
and bined with TTT treatment V) animal models were induced with semiconductor weeks later,the histological changes of retina,choroid of rabbits were observed and the area and MOD of axon in inner plexiform layer of retina were measured under light :The area and MOD of axon in bined with TTT treatment group were significantly higher than those in TTT treatment and TCM treatment :Guishao Dihuang bined with TTT has a better therapeutic effect for age-related macula degeneration.
Key words age-related macula degeneration,choroidal neovascularization,transpupillary thermotherapy,neuroprotective effect 年龄相关性黄斑变性(AMD)属于祖国医学“视瞻昏渺”的范畴,现