SMAF1基因的异位过表达对脂肪细胞3T3L1增殖和凋亡的影响.doc

SMAF1基因的异位过表达对脂肪细胞3T3L1增殖和凋亡的影响
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作者:韩威段佳慧吴民泸林泽超刘彦华
【摘要】目的观察一脂肪细胞特异性表达的新基因小脂肪细胞因子1(SMAF1)在小鼠脂肪前体细胞3T3L1中的异位表达对脂肪细胞增殖和凋亡的影响。方法构建小鼠SMAF1真核表达载体并稳定转染3T3L1脂肪前体细胞以获得永久异位表达SMAF1基因的细胞株,使用Western blot证实SMAF1基因的表达。体外培养SMAF13T3L1脂肪细胞,以MTT法检测SMAF13T3L1脂肪细胞的增殖;用流式细胞仪对SMAF13T3L1脂肪细胞进行凋亡分析。结果与对照相比,SMAF13T3L1脂肪细胞增殖明显降低,差异有统计学意义();而SMAF13T3L1脂肪细胞凋亡分析结果与对照无显著性差异。结论 SMAF1基因在3T3L1中的异位性表达,对3T3L1前体脂肪细胞生长增殖有显著的抑制作用,而对细胞凋亡无影响,提示SMAF1在脂肪细胞增殖分化中发挥一定的作用。
【关键词】 SMAF1; 脂肪细胞; 异位表达; 增殖; 凋亡
【Abstract】 Objective To evaluate the effect of a novel adipocyte
specific expressed gene small adipocyte factor 1 (SMAF1) on proliferation and apoptosis of preadipcyte 3T3L1. Methods A permanent SMAF1 expression construct was established for stable transfection of 3T3L1 and formation of SMAF1 3T3L1 cell line. After invitro culturing of cells,both MTT method and flow cytometry were used to detect and analyze the proliferation level and apoptosis progress respectively. pared with control groups,SMAF1 3T3L1 cell line showed remarkable reduction in proliferation with statistical significance (),while no notable difference in apoptosis between two cell lines. Conclusion The ectopic expression of SMAF1 in 3T3L1 preadipocyte might suppress proliferation without interfering apoptosis process.【Key words】 small adipocyte factor 1; preadipocytes; ectopic ex