T细胞亚群、IL-2、NSE与新生儿HIE的研究及临床意义.doc

T细胞亚群、IL-2、NSE与新生儿HIE的研究及临床意义
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作者:郑振文李吉昌李桂英王玉珍董秀梅韩兆东
【摘要】目的探讨新生儿缺氧缺血性脑病(HIE)外周血T细胞亚群、IL-2及神经元特异性烯醇化酶(NSE)的动态变化,为新生儿HIE的诊断、分度、治疗及预后提供新的理论依据。方法选择36例围生期有缺氧缺血性脑病的新生儿为研究对象(HIE组),同时选择20例同期出生的正常足月儿为对照组,分别检测T细胞亚群CD3、CD4、CD8、CD4/CD8比值、IL-2和血清NSE的动态变化。结果①急性期HIE组T细胞亚群CD3、CD4、CD4/CD8比值及IL-2水平低于正常对照组(P<),CD8及NSE水平高于对照组(P<);②不同程度HIE组急性期T细胞亚群、IL-2及NSE水平之间相比有显著差异性(P<);③恢复期T细胞亚群、IL-2及NSE水平与正常对照组之间相比仍有显著性差异(P<),和急性期相比随病情的好转各项指标有明显改善(P<);④T细胞亚群CD3、CD4、CD4/CD8比值、IL-2与 NSE呈负相关,CD8T细胞亚群与NSE呈正相关。结论①新生儿HIE存在着免疫功能紊乱;②T细胞亚群、IL-2及NSE参与了新生儿HIE的病理生理过程;③外周血CD8T细胞亚群和NSE水平升高,CD4/CD8比值和IL-2水平下调,与新生儿HIE轻重程度呈正相关。
【关键词】缺氧缺血性脑病;T细胞亚群;神经元特异性烯醇化酶;IL-2;新生儿
【Abstract】 Objective To evaluate the dynamic changes of T cell subgroups, IL-2 and NSE levels in the newborn babies with HIE and its clinical significance, and to provide new evidence for the diagnosis, therapy and prognosis of To select 36 cases of infants with HIE in perinatal period and they were divided into three groups depending on the severity of the patients’ condition, and at the same time to select 20 cases of healthy newborn babies as control group. To collect peripheral venous blood in acute stage and restoration stage of the HIE group .Two blood samples were collected in each cases, one is on the first to third day after birth, the other on the seventh to tenth day af