VEGF和MVD在乳腺疾病血管生成中的意义.doc

VEGF和MVD在乳腺疾病血管生成中的意义
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作者:武变荣李积德郭振昌骆玉霜沈存芳
【摘要】目的探讨VEGF和MVD在乳腺疾病血管生成中的意义。方法采用免疫组化染色法(S-P法)检测70例乳腺疾病患者(乳腺良性疾病患者28例,乳腺癌患者42例)术后标本中VEGF表达水平和MVD值。结果乳腺癌组VEGF高表达比例和MVD值均较乳腺良性疾病组高,差异有统计学意义();乳腺癌组VEGF表达与年龄、肿块大小、HER2相关,与腋淋巴结、临床分期、ER、PR无关,而MVD仅与肿瘤大小和HER2相关。VEGF高表达组MVD值显著高于VEGF低表达组MVD值,差异有统计学意义()。结论联合检测VEGF和MVD对预测乳腺疾病预后有积极意义。
【关键词】乳腺癌 VEGF MVD 免疫组化染色法
Abstract Objective To detect the value of MVD and VEGF in breast disease angiogenesis. Methods The expression of VEGF and MVD in 70 cases of breast diseases was examined by immunohistochemistry staining (SP-method). Results VEGF expression and MVD value in breast cancer were much higher than benign breast disease, there was a correlation between VEGF expression and age, tumor size and HER2 in breast cancer and there was no correlation between other clinical parameters, but MVD was correlated with tumor size and HER2. MVD valve in VEGF high expression group was higher than VEGF low expression group (P ). Conclusion It shows positive significance in detection of MVD and VEGF both in breast disease angiogenesis.
Key words Breast Cancer VEGF MVD Immunohistochemistry staining
乳腺癌与所有实体肿瘤一样, 其肿瘤细胞旺盛的代谢、增殖需要充足的血液供应, 其肿瘤新生血管形成与肿瘤的发生、发展密切相关。促血管形成因子的表达能促进肿瘤新生血管形成, 为肿瘤的生长、浸润及转移创造条件,血管内皮生长因子(VEGF)是已知作用最强、活性最高的促血管形成因子;微血管密度(MVD)是显示肿瘤血管形成活性的重要指标。我们分别应用抗Ⅷ因子抗体和VEGF单克隆抗体染色免疫组化方法检测乳腺疾病患者病例中M