北美刺人参指纹图谱及与东北刺人参化学成分比较研究.doc

北美刺人参指纹图谱及与东北刺人参化学成分比较研究
作者:赵月然,康廷国,窦德强,张放,王首千
【摘要】目的研究北美刺人参(Oplopanax horridus,OH)的HPLC指纹图谱,为科学评价及有效控制药材质量提供可靠方法。,色谱条件为:色谱柱Kromasil C18 column(250 mm× mm,5 μm),预柱Zorbax( mm× mm,5 μm)流动相为乙腈-% 磷酸水溶液梯度洗脱, ml/min,柱温为 35℃,检测波长210 nm。对11批茎皮药材进行了相似度分析,并同东北刺人参(O. elatus Nakai,OE)茎皮指纹图谱进行了比较,采用系统聚类分析方法处理数据。结果相同种类药材的相似度较高,,采用聚类分析可以聚为一类。结论指纹图谱可用于刺人参药材不同种类的鉴别。
【关键词】北美刺人参; 东北刺人参; 指纹图谱; 聚类分析
AbstractObjectiveTo study on the HPLC fingerprints of Oplopanax horridus for offering a method on the quality control and assessment. MethodsSyringenin was used as reference substance to establish HPLC fingerprints of stem bark of O. horridus. Separations and determinat
ions were carried out by linear gradient elution under following conditions: Kromasil C18 column(250 mm× mm,5 μm),pre-column Zorbax( mm× mm,5 μm), acetonitrile- % phosphoric acid solution as mobile phase, detection wavelength of UV at 210nm, flow-rate of ml/min, column temperature of 35℃. Similarity calculation software was used to evaluate the whole HPLC spectrums of 11 samples of OH stem bark. Furthermore, it pared with fingerprint of O. elatus Nakai stem bark and clustering analysis was applied for data analysis. ResultsThe similarity of HPLC fingerprint was more than among the same types of fingerprint can be used for d