细胞趋化因子受体5在病毒性肝炎患者外周血单核细胞中的表达.doc

细胞趋化因子受体5在病毒性肝炎患者外周血单核细胞中的表达
作者:颜学兵,张萍,吴文漪,冯霞,何静
【关键词】细胞
摘要:目的 R5)在乙型病毒性肝炎(HB)免疫发病机制中的作用。方法用流式细胞仪对20例正常人(NC)及20例HB患者外周血单核细胞(PBMC)水平进行检测,并比较其经植物凝血素(PHA)培养前后的变化。结果 R5的表达无明显差异(P>);R5表达水平高于NC(P<);R5表达均较培养前明显减少(P<),R5表达虽较培养前减少,但无统计学意义(P>);急性乙型肝炎(AHB)、慢性乙型肝炎(CHB)、肝硬化(LC)R5的表达有一定的差别:AHB>CHB>LC,但无统计学意义(P>)。R5的表达明显低于阴性组(P<)。结论 R5的表达水平与HB的临床转归可能有一定的关系,R5患者预后较好;R5的表达可能不利于乙型肝炎病毒(HBV)的复制。

关键词:乙型肝炎;发病机制;乙型肝炎病毒;细胞趋化因子受体5

Abstract:Objective To investigate the role chemokine R5)in the immunological pathogenesis of hepatitis B(HB).Methods The expression levels R5in peripheral blood mononuclear cell(PBMC)were assayed by flow cytometry before and after PHA-induced cultivation in20HB patients and20normal controls(NC).Results Before cultivation,there was no discrepancy R5level between NC and HB(P>).After R5was obviously lowered in NC group(P<),but insignificantly in HB group(P>),with the slightly R5levels in an or-der as AHB>CHB> was also noticed that R5level was lower in the HB patients positive for HBV duplicating markers than that in those who were negative(P<).R5expression level in PMBC may be related to the e of HB,and higher levels R5suggest relatively favorable prognosis and limit HBV replication.

Key words:hepatitis B;pathogenesis;hepatitis B chemokine receptor5

趋化性细胞因子是细胞因子的