西藏小型猪ESR基因PvuⅡ多态性与生长繁殖性能相关分析.doc

西藏小型猪ESR基因PvuⅡ多态性与生长繁殖性能相关分析
作者:曾昭智江涛张锦红顾为望
【摘要】目的了解ESR基因PvuⅡ位点在西藏小型猪中的多态性分布情况;研究其在西藏小型猪上的遗传效应,为西藏小型猪生长繁殖性状的分子遗传标记辅助选择提供实验依据。方法记录44头西藏小型猪母猪繁殖性能,并对其所产仔猪的生长性能进行测定,采用PCRRFLP方法对ESR基因PvuⅡ位点进行多态性与生长繁殖性能进行相关性分析。结果西藏小型猪ESR候选基因PvuⅡ位点表现出多态性,为AA、AB、BB型,A、B等位基因频率特征。初产胎的产仔数在不同基因型间差异存在显著性,不同基因型间产仔数呈BB>AB>AA的趋势,初产胎和经产胎的母猪其他指标在不同基因型间差异均没有显著性。结论提高西藏小型猪猪群ESR基因PvuⅡ位点B等位基因频率,有利于提高西藏小型母猪的产仔数。
【关键词】西藏小型猪 ESR基因多态性生长繁殖性能相关分析
Abstract:Objective To obtain the information on PvuⅡpolymorphism distribution of ESR gene and study its ic effect on Tibet minipigs to provide experimental evidence for molecule ic marker selection of growth and reproductive traits. Methods Growth and reproduction traits of 44 Tibet minipigs were recorded. The PvuⅡ polymorphism of ESR gene was detected by PCRRFLP and the relationship between polymorphism and growth and reproduction performance was analyzed. Results The genotypes were AA, AB, BB with A and B were alleles. TBN(total birth numb
er)of the primiparous sows was significantly different between different genotypes and the tendency of TBN between different genotypes was BB>AB>AA, other index between primiparous sows and parous sows showed no significant difference. Conclusion The study results demonstrated that improving allele frequency of B allele of ESR gene might be helpful to improve TBN of Tibet minipigs.
Key words:Tibet minipig; ESR gene