间歇性缺氧对幼年大鼠大脑皮层电活动的影响.doc

间歇性缺氧对幼年大鼠大脑皮层电活动的影响
作者:罗斌;陈燕赵春玲,罗礼容
【摘要】目的观察间歇性缺氧损伤后幼鼠前额叶皮层电活动的变化。方法 SD雄性大鼠60只,20±2日龄,随机分为6组:正常2周组、正常4周组、正常6周组、间歇性缺氧2周组、间歇性缺氧4周组、间歇性缺氧6周组,每组10只。正常(UC)组正常饲养,间歇缺氧(IH)组建立间歇性缺氧损伤模型。用金属电极记录皮层放电活动。结果间歇性缺氧2周后,大鼠前额叶皮层δ波、θ波所占比例较正常组增多,α波、β波所占比例较正常组减少,有显著性差异(P<);而间歇性缺氧4周和6周后,虽然大鼠前额叶皮层δ波、θ波所占比例较正常组有所增多,α波、β波所占比例较正常组有所减少,但均未达到显著性差异(P>)。结论随着幼鼠年龄的增加,神经系统发育成熟,从而使间歇性缺氧所造成的脑损伤得到一定的修复,脑损伤程度减轻,最终使前额叶皮层电活动发生的异常变化逐渐减弱。
【关键词】间歇性缺氧;发育;脑电图;阻塞性睡眠呼吸暂停低通气综合征;脑损伤;GAP-43
Abstract Objective To observe the electrical activity changes of the prefrontal cortex of the neonate rats after intermittent hypoxia. Methods 60 SD male rats, 20
±2 days old, were randomly divided into 6 groups: unhandled control 2-week group, unhandled control 4-week group, unhandled control 6-week group, intermittent hypoxia 2-week group, intermittent hypoxia 4-week group and intermittent hypoxia 6-week group, 10 rats in each; normal feeding was given to the rats in the unhandled groups while the models of intermittent hypoxia injury were set up in the intermittent hypoxia groups; mental electrodes were used to record the electrical activity of the After 2 weeks of intermittent hypoxia, the proportions of δ and ο wave in rate prefrontal cortex in intermittent hypoxia groups were bigger than those in unhandled control groups while the proportions of α and β wave were smaller than those in unhandled co