2，6-双（3’-溴甲基-1’-吡唑基）-4-溴吡啶的合成与表征.pdf.pdf

,6-双(3-溴甲基-1-吡唑基)-4-溴吡啶的合成与表征石竹,李宏涛,刘婷婷,潘利华,赫文龙(,占林长春130012;,吉林长春130022)摘要:以2,6-双(3'-甲基一1一吡唑基)4一氨基吡啶为原料,经重氮化,溴化合成了新型时间分辨荧光免疫分析双功能螯合剂中间体2,6-双(3溴甲基一1一吡唑基),MS,’HNMR和元素分析进行了表征,:荧光免疫分析;2,6一双(3一溴甲基一1一吡唑基)4一溴吡啶;螯合剂中图分类号::A文章编号:1008—1011(2008)01—0052—03SynthesisandCharacterizationof2,6-Bis()-,LIHong—tao,LIUTing—ting,PANLi—hua。HEWen—long(,Changchun130012,Jilin,China;,ChangchunInstituteofAppliedChemistryChineseAcademyofSciences,Chaagchun130022,Jilin,China)Abstract:Anewbifunctionalchelateintermediateoftime—(3一bromomethyl—pyrazol一1一y1)-4一bromopyridinewaspreparedfrom2,6-bis(3一methyl一1H—pyrazol一1一y1)-4一aminopyridinebydiazotization——uctwasdeterminedbyIR,HNMR,—:fluorescenceimmunoassay;2,6一bis(3一bromomethyl—pyrazol一1一y1)-4一bromopyridine;che—lat时间分辨荧光法与长寿命微秒级镧系标记物的结合¨卫J,,它量子产率高,Stokes位移大,发射峰窄,激发和发射波长理想,荧光寿命长(10~1000p,s).采用时间分辨技术,可消除激发光源的光、电干扰及样品池和样品本身等发出的短寿命荧光(1~10ns),,2,