HPLC法测定茜草中大叶茜草素、羟基茜草素含量.doc


HPLC 法测定茜草中大叶茜草素、羟基茜草
素含量#
林文华,朱喜梅,严萍,詹若挺,刘翠婷,张洁**
5
10
(广州中医药大学中药资源科学与工程研究中心岭南中药资源教育部重点实验室,广州
510006)
摘要:【目的】建立茜草中大叶茜草素、羟基茜草素的 HPLC 含量测定方法。【方法】采用
LUBEX Ecosil C18 柱( mm×250 mm,5 μm),流动相为甲醇-乙腈-%磷酸水(25:
50: 25),流速 mL· min-1,检测波长为 250 nm。【结果】大叶茜草素和羟基茜草素分
别在 ~ μg·
mL-1(r2=)和 ~ μg·
mL-1(r2=)
范围内呈良好线性,平均回收率分别为 %(RSD=%)和 %(RSD=%)。
【结论】该方法操作简单,重复性好,可作为茜草中大叶茜草素和羟基茜草素的含量测定方
法,为合理制定茜草的含测方法提供参考。
15
20
25
关键词:茜草;大叶茜草素;羟基茜草素;含量测定
中图分类号:
HPLC determination of rubimaillin and purpurin in Rubia
cordifolia L.
LIN Wenhua, ZHU Ximei, YAN Ping, ZHAN Ruoting, LIU Cuiting, ZHANG Jie
(Research Center of Chinese Herbal Resource and Engineering, Key Laboratory of Lingnan
Chinese Medicinal Resource, Ministry of Education, Guangzhou University of Chinese Medicine,
Guangzhou 510006)
Abstract: Objective To establish a HPLC method to determine the content of rubimaillin and
purpurin in Rubia cordifolia L.. Method LUBEX Ecosil C18 column( mm×250 mm,5 μ
m)was used with methanol- acetonitrile- % phosphoric acid solution as the mobile phase, the
flow rate was mL·
min-1 and the PDA detective wavelength was set at 250 nm. Result The
rubimaillin had a good linearity relationship between - μ g·
mL-1(r2=),
the purpurin showed a good linearity relationship whithin the range of 1