丁玉强－形态学.ppt

Electronmicroscope&InsituhybridizationLabofNeuralDevelopment,ION丁玉强E-mail:******@ElectronMicroscopyBriefintroductionofEMForexaminationofultra-structuresatsubcellularlevel(Membrane,Lysosome,Golgiapparatus,Synapse)ThesameapproachaslightmicroscopySection:mvs60nm(ultrathinsection)Lightwave(-m)vselectron“wave”(2x10-5m)Resolution:>mvsnmImages:filmanddigitalCategoriesinBiologyTransmissionEM透射电镜ScanningEM扫描电镜TransmissionEM光源:电子枪,超高压,电子束注:在使用时,电镜镜体内保持真空状态。抽真空需要若干小时成像系统:电磁场,会聚成像观察系统:荧光屏,胶片,数码设备JustshowyouseveralexamplesandthengiveyouabriefintroductionofhowtodoitRoughERSmoothERGolgiapparatusSecretarygranuleEndosomeMultivesicularbodyPrimarylysosomeSecondarylysosomeTertiarylysosomeSynapseMyelinatedfiberRanviernodeFixation:GlutaraldehydeisrequiredPost-fixation:1%:-