二烯丙基三硫通过线粒体依赖性途径诱导人肝癌HepG2细胞凋亡.pdf

目录一主要缩略语英文索引??.二中文摘要????一?一~一??????????????._4三英文摘要????一?一??????一????????一一??一一???一一????????.??????一一??一一???一一??????????????????????????一????????一???一???一一????一?????????一??????一????????????????????一???一?一????????~??????一?????一??????一~一25五参考文献??一???一????????????????26六综述???一?????一???????????????32七硕士期间撰写的论文?一????????????一???41八致谢????一一??一???~?????一一?????422DATSCyt-c主要英文缩略语索引DiallyltrisulfideCytochrome—aspase一3Cysteinylaspartate—specific半胱天冬酶3AymAo/EBSDSPAGEPBSFBSDMS0PMSFSPSSprotease3Mitochondrialmembranepotential线粒体膜电势Acridineorange/ethidiumbromide丫啶橙/溴化乙锭十二烷基硫酸钠Polyacrylamidegelelectrophroesis聚丙烯酰***凝胶电泳Fetalbullserum磷酸盐缓冲液胎牛血清Dimethylsulfoxid二***亚砜phenylmethylsulfonylfluoride苯***磺酰***Statisticalpackageof社会科学统计软件包socialscience3二烯丙基三硫通过线粒体依赖性途径诱导人肝癌HepG2细胞凋亡硕士研究生:肖志科指导教师:唐圣松教授姜浩教授中文摘要目的:探讨线粒体在二烯丙基三硫(diallylIrisulfide,DATS)诱导人肝癌细胞系HepG2细胞凋亡过程的作用。方法:用AO/EB染色法观察细胞凋亡情况,在显微镜下计数凋亡细胞数;;;Elase法检测caspase-3活性。结果:DATS诱导HepG2细胞凋亡,用501arnol/L与1001xmoULDATS处理48小时,%%,并引起HepG2细胞线粒体膜电势降低;westerllblot实验显示:,与此同时,线粒体细胞色素一C减少,诱导HepG2细胞caspase一3活化。结论:DATS通过降低线粒体膜电势,促进细胞色素C由线粒体膜释放到胞浆中,激活caspase-3途径诱导人肝癌细胞系HepG2细胞凋亡。关键词:HepG2细胞;线粒体;二烯丙基三硫;凋亡学科分类号:-dependentPathwayABSTRACTObjective:ToexploretheeffectofDATSonhumanhepatocellularcarcinomaHepG2cellapoptosisandtheroleofmitochonddainDATS—:HepG2cellswereeithertreatedwith50ttM,I00肛MofDATSfor0,6,12,24,---:%%,respectively,