神秘果果实总RNA提取方法比较研究.pdf

基因组学与应用生物学,2013年,第32卷,第3期,第409-412页Genomics and Applied Biology, 2013, , , 409-412技术专题Technology Feature神秘果果实总RNA提取方法比较研究谭才邓1,2*李静1,2邓毛程1,21广东轻工职业技术学院,广州, 510300; 2广东高校特色调味品工程技术开发中心,广州, 510300*通讯作者, tcdeng@摘要以神秘果果实为材料,以获得高质量RNA为目的,以针对性地去除多糖、酚类物质为出发点,比较了Trizol法、CTAB法,并相应加以改良。结果显示:CTAB粗提法方便快捷,CTAB精提法提取的总RNA完整性好、纯度高,RT-PCR反应表明提取的总RNA都能满足分子生物学实验的要求。关键词神秘果,果实, RNA提取Study on Total RNAExtraction Methods fromMiracle FruitTan Caideng1,2*Li Jing1,2Deng Maocheng1,21 Guangdong Industry Technical College, Guangzhou, 510300; 2 Centre of Guangdong Higher Education for Engineering and TechnologicalDevelopment of Speciality Condiments, Guangzhou, 510300* Corresponding author, ******@DOI: .000409AbstractIn order to extract total RNA form miracle fruit and reduce the influence of harides andpolyphenolics, we studied pared four RNA extraction methods such as Trizol method, Trizol improvedmethod, CTAB crude method and CTAB refined method. The results showed that: The CTAB crude extractionmethod was convenient and quick, total RANfromthe CTAB refined method was proved to be relatively intact andhigh purity, RT-PCR analysis showed that the total RNA from both CTAB methods could be used in furthermolecular fruit, Fruit, RNAisolation基金项目:本研究由“广东高校特色调味品工程技术开发中心”开放课题项目(GCZX-B1103)资助神秘果(Synsepalum dulcificum)属于山榄科神秘果属,原产西非,自20世纪60年代引入中国,在海南、广东、广西等