头花蓼和头状蓼的HPLC指纹图谱鉴定研究.pdf.pdf

天然产物研究与开发NatProdesDev,2007,19:253-255文章编号:1001-6880(2007)Suppl-0253-03头花蓼和头状蓼的HPLC指纹图谱鉴定研究2,百1佶,~J业bh,裴瑾,吴红梅成都中医药大学药学院,成都611730;贵阳中医学院,贵阳550002摘要:建立头花蓼、头状蓼HPLC指纹图谱鉴定方法。(DAD)法,梯度洗脱,测定不同产地头花蓼及头状蓼的指纹图谱,并作相似度比较分析。不同产地头花蓼指纹图谱相似度较好,而头花蓼与头状蓼的指纹图谱有明显差异。HPLC指纹图谱具有重现性好,特征性强,方法简便等特点,可用于头花蓼、头状蓼药材的鉴别。关键词:头花蓼;头状蓼;高效液相色谱;指纹图谱中图分类号::AIdentificationofHerbPolygonumcapitatumandHerbPolygonumnepalensebyHPLCFingerprintWANGXiang—pei’,’,PEIJin,WUHong—meiPharmaceuticalCollege,ChengduUniversityofTraditionalChineseMedicine,Chengdu611730,China;GuiyangCollegeofTraditionalChineseMedicine,Guiyang550002,ChinaAbstract:ToestablishHPLCfingerprintfortheindentifieationofherbPolygonumcapitatumandherbPolygonumne·pa~e/'/,$e,chromatographicfingerprintofPolygonumcapitatumfromdifferentproducinga1~a8andherbPolygonumne·p~/e/'/,$eweredeterminedbyRP—HPLC(DAD)andthegradientelutionmode印puedinchromatographicseparation,~a8wereofhighsimilarity,~:herbPolygonumcaphutum;herbPolygonumnepalense;HPLC;,具有清热利湿,解毒止痛,和血散瘀,利尿通淋之功效;头状蓼为蓼科植物尼泊尔蓼Polygonum聊一palenseMei