l4致肝纤维化大鼠肝组织TGFTβRⅠ和TβRⅡ的影响.pdf

陕西中医学院硕士学位论文 l4致肝纤维化大鼠肝组织TGFTβRⅠ和TβRⅡ的影响姓名:吴兰申请学位级别:硕士专业:中医基础理论指导教师:黄广平 2011-04 中文摘要目的:l 4致肝纤维化大鼠肝组织转化生长因子β1受体Ⅰ(TβRⅠ) 和转化生长因子β1受体Ⅱ(TβRⅡ)表达的影响,探讨其抗肝纤维化的疗效及作用机理。 方法:选用SD 雄性大鼠70 只,l 4)植物油溶液皮下注射加酒精喂饲等复合方法制备肝纤维化大鼠模型,设模型组、肝复康小、中、大剂量组及复方鳖甲软肝片组,各组均给与灌胃治疗,连续用药8 周。实验结束后处死各组大鼠,观察肝脏形态,测肝脏体重,计算肝脏指数;检测血清ALT、AST 的含量,并对肝组织病理切片采用苏木精-伊红染色(HE 染色) ;检测肝组织变性程度及肝纤维化程度;用免疫组化方法(SP法)检测TβRⅠ和TβRⅡ的含量。 结果:与正常组比较,模型组大鼠肝组织TβRⅠ和 TβRⅡ的表达显著升高,差异有显著性( P< );肝复康小剂量组与模型组比较,大鼠肝组织TβRⅠ和TβRⅡ的表达无显著性差异( P>);肝复康大、中剂量组、复方鳖甲软肝片组均与模型组比较,大鼠肝组织TβRⅠ和TβRⅡ的表达显著降低,差异有显著性( P<),但三组间均无显著性差异( P >)。 结论:Ⅰ、TβRⅡ的表达,影响受体与 TGF-β1 的结合,进而促进肝内细胞外基质(ECM)降解,l 4 )诱导的肝纤维化大鼠具有良好的治疗作用。 、中剂量组在抑制HF 大鼠肝组织中TβRⅠ和TβRⅡ的效果与复方鳖甲软肝片基本相同。 。 关键词肝复康;肝纤维化;TβRⅠ;TβRⅡ;免疫组化 Effect of GFK Decoction T β R Ⅰ and T β R Ⅱ on Hepatic Tissue of Hepatic Fibrosis Rat L 4 Abstract Objective : To observe the effect on hepatic tissue transforming growth f actor β1 recepto rⅠ(TβRⅠ) and transforming growth factor β1 receptor Ⅱ(T βRⅡ) of hepatic fibrosis in rats by GFK ,and further discuss th e mechanism of anti-action and the appropriate dosage. Methods :SD male rats, using carbon l 4) subcutaneous injection with ethanl fed,preparation of liver fibrosis model was set up in pathological model group,GFK in small, medium and large dose group po und FFBJRGP group ,after a essful modeling gavage treatment,were treat for 8 weeks. After treatment,rats in each group were sacrificed,observation liver shape,measure liver weight, calculation liver index,check conte nt of blood serum ALT,AST,and their liver biopsy for HE stainingto observe the extent of liver fibrosis. all the rats were exected and their lives were obtained for the content of hepatic tisse T βRⅠand TβRⅡ were detect by immunohisto chemical technique. Results : TβRⅠand TβRⅡ of the model group inceased significant,which compared with the normal group( P<).The low GFK group has no obvious difference,pared with the mo