该论文来源于网络,本站转载的论文均是优质论文,供学****和研究使用,文中立场与本网站无关,版权和著作权归原作者所有,如有不愿意被转载的情况,请通知我们删除已转载的信息,如果需要分享,请保留本段说明。 【摘要】 目的:探索一種可行的男科实验室计算机辅助精液分析系统(Computer-aided Semen Analysis System,CASA)的质控方法。方法:参考第5版《世界卫生组织人类精液分析实验室技术手册》推荐的分析方法和质控标准,分别使用高、低浓度的自制精液质控品和商品化质控珠对CASA的检测结果进行一致性分析。同时由两名实验员使用CASA对18份精液样本的浓度和活力进行双盲分析,并绘制Bland-Altman图对检测结果进行比较分析。结果:自制精液质控品高、%、%,质控珠高、%、%,均符合Levey-Jennings质控规则。Youden分析显示两种质控品的检测结果均位于95%CI。Bland-Altman图分析显示,两名实验员对18份精液标本的浓度和活力检测结果差值的绝对值均位于95%CI内,检测结果一致性高。结论:CASA需要适当的质控方案保证其分析结果的准确性,本研究中无论是自制的精液质控品还是商品化质控珠,均能在CASA的精液浓度质控中起到良好的效果。在精子活力分析质控方面,CASA比传统的人工分析方法能够更加准确捕捉精子运动轨迹并提供更多精子运动信息。男科实验室的CASA质量控制不仅需要严格执行室内质控,还需要通过室间质评进一步保证仪器检测结果的准确性。 【关键词】 计算机辅助精液分析 质量保证 质量控制 Application and Quality Control of Computer-aided Semen Analysis System in the Andrology Laboratory/WANG Cheng, FAN Shushu, ZHANG Si, HUANG Wenbo, HOU Zhiwei. //Medical Innovation of China, 2020, 17(13): -148 [Abstract] Objective: To explore a feasible method for quality control of computer-aided semen analysis (CASA) in the andrology laboratory. Method: With reference to the analytical methods and standards recommended in the 5th edition of WHO Laboratory Manual for the Examination and Processing of Human Semen, the consistency analysis of CASA system was conducted by using high and low concentrations of self-made semen quality control samples and commercial quality control beads. At the same time, the concentration and motility of 18 different semen samples were double-blind analyzed by two experimenters using CASA system, then the bland-altman graphs was drawn to compare the test results. Result: The variation coefficients of high and low concentration self-made semen quality control samples were % and %, respectively. The intra-batch variation coefficients of high and low concentration control beads were % and %, respectively. A