HLA-DRB1、DQB1基因多态性与新疆哈萨克族结核病易感性的相关性研究.pdf

石河子大学
硕士学位论文
HLA-DRB1、DQB1基因多态性与新疆哈萨克族结核病易感性的相
关性研究
姓名:贺鹏
申请学位级别:硕士
专业:病理学与病理生理学
指导教师:张万江
20100501
摘要
目的:探讨人类白细胞抗原(human leukocyte antigen, HLA) -DRB1、DQB1 基因多态性与新疆哈
萨克族人群结核病(TB)易感性的相关性。
方法: (1)采用病例-对照的研究方法,以 231 例新疆哈萨克族结核病患者和 230 例同民族健康对照者
为研究对象。(2)应用聚合酶链反应-序列特异性引物(Polymerase chain reaction-sequence specific
primers, PCR-SSP) 技术对 HLA-DRB1、-DQB1 基因进行分型,比较组间等位基因频率(GF) 并计算其
比值比(OR)。(3)应用 统计软件进行统计学处理,采用卡方检验,对两组资料的 HLA-DRB1
及 DQB1 等位基因频率的差异,分别进行统计学分析(P<)。
结果:(1)在 HLA-DRB1 等位基因中,实验组与对照组的 HLA-DRB1*04 等位基因的基因频率分别
为 %和 %, p<0·05, OR=, 有统计学意义;HLA-DRB1*10 也增高(%比 %), 但统
计学上无显著性差异(P>0·05)。(2)在 HLA-DQB1 等位基因中,实验组和对照组的 HLA-DQB1*0201
等位基因的基因频率分别为 %、%, P<, 组间差异有统计学意义,OR 值为 .
结论:(1) HLA-DRB1*04 可能是新疆哈萨克族人群结核病的易感基因。(2) HLA-DQB1*0201 可能是
新疆哈萨克族人群结核病的易感基因。


关键词: HLA 基因,基因多态性,新疆哈萨克族,结核病,易感性
论文类型:A(基础研究)











I
Abstract
Objective: To investigate the association between the polymorphism of HLA-DRB1gene、HLA-DQB1
gene and Susceptibility to tuberculosis in Xinjiang Kazaks Population.
.Methods: (1)Using case- control study, we selected 231 tuberculosis cases and 230 controls Kazaks
population from Xinjiang provinces. (2)The polymorphism of HLA-DRB1 gene and HLA-DQB1 gene in
the two groups were detected with polymerase chain reaction-sequence specific primers(PCR-SSP)
technique, Gene frequency (GF) and odds ratio ( OR) were calculated pared.. (3)The
was used, to analyze the difference between the frequency of HLA-DRB1 and HLA-DQB1 alleles of the 2
groups by chi square test.(P<)
Results: (1)The frequency of allele HLA-DRB1*04 of cases are %, and the same alleles of controls
are %, p<. OR=, there are significant differences of alleles HLA-DRB1*04 between the 2
groups; The frequency of DRB1*10 was also increased, but the difference was not statistically
significant(% vs %; P>). (2)The frequency of allele HLA-