医学论文英文摘要 V.ppt.ppt

Lectures on Writing Medical Paper Abstracts in English 第五讲 SCI 收录我国三种医学杂志英文摘要赏析 SCI 是美国《科学引文索引》( Science Citation Index )的缩写。由于 SCI 数据库具有学科全面、学术影响大、覆盖国家广泛等特点, 因而被其收录检索的期刊和论文分别被称作国际主流期刊和国际主流科学。刊物或论文被 SCI 收录和引用在一定程度上反映了该刊物或论文具有较高的学术水平和较大的国际影响力。我国目前被 SCI 收录的科技期刊共 60种,而临床医学类只有中华医学杂志(英文版)、中国药理学报和世界胃肠病杂志 3种。一、中国药理学报( Acta Pharmacologica Sinica ) Sample 1 Acta Pharmacologica Sinica 2007 February; 28 (2): 238-245 Effect of genistein on mouse blastocyst development in vitro Abstract Aim: 1) To examine the cytotoxic effects of genistein , an isoflavone compound, on early postimplantation embryonic development in vitro . Methods: 2) Mouse blastocysts were incubated in medium with or without genistein (25 or 50 μ mol/L) or daidzein (50 μ mol/L) for 24 h. 3) Cell proliferation and growth was investigated by dual differential staining, 4) apoptosis was analyzed by terminal deoxynucleotidyl transferase -mediated dUTP nick-end labeling (TUNEL) assay, and 5) apoptotic or necrotic cells were visualized by Annexin -V and propidium iodide (PI) staining. 6) Implantation and postimplantation development of embryos were measured by in vitro development analysis Results: 7) TUNEL staining and Annexin -V/PI staining showed that genistein dose-dependently increased apoptosis in mouse blastocysts , while daidzein , another soy isoflavone , had no such effect. 8) The pretreatment of the blastocysts with genistein caused fewer cells than the control group and this effect was primary in the inner cell mass. 9) The genistein -pretreated blastocysts showed normal levels of implantation on culture dishes in vitro , but significantly fewer genistein -pretreated embryos reached the later stages of embryonic development versus the controls, with many of the former embryos dying at relatively early stages of development. In addition, 10) genistein treatment decreased the development of morulas into blastocysts , and dietary