《3,5-二硝基水杨酸比色法测定红毛五加中多糖的含量》.pdf.pdf

3,5一二硝基水杨酸比色法测定红毛五加中多糖的含量章丽华1,张利平2胡锦群1,冯丽娟1,张龙清1,张莅峡3 (,浙江金华321017,,浙江丽水323000: ,北京1 00700) 摘要:目的建立红毛五加中多糖的含量测定方法。方法采用3,5一二硝基水杨酸比色法(DNS法),测定红毛五加中的还原糖和总糖的含量,并计算出总多糖的含量。结果以葡萄糖为对照品,— mg/L(,· ),%~%;— mg/、稳定、重复性好,有助于红毛五加质量控制方法的研究. 关键词:红毛五加多糖;葡萄糖;3,5一二***水杨酸比色法中图分类号: 文献标识码:A 文章编号:1005—5304(2008)05—0049-03 Quantitative Detemination ofAGP by DNS Method ZHANG ,ZHANG Li—pin92,HU Jin-qunl,et Asia Pharmacy Ltd.,Jinhua 3210I L China; Municipal Hospital,Lishui 323004 China) Abstract:Objective To establish amethod forthequantitative determination ofAGP{Acanthopanax 舀raldij Harms harideS).Methods AGP content was determined by DNS absorptions of processed samples weretested atthewavelength of520 Contrast toglucose,the linear range was ~ mg/L(r= 2),the average recovery was %~%,RSD=%,凡= harides measured in3batches ofsamples were ~ Themethod issensitive。 steadywith good repeatability,and help tothequn秒controlofAcanthopanax百谢dii Harms. Keywords:Acanthopanax giramii Harms harides(AGP);glucose;DNS method 红毛五加(Acanthopanax giraldii Harms)是五加科五加木脂素类、核苷类、多糖类、氨基酸、有机酸及微量元素等多属植物,主产于我国四川省““。四川省的红毛五加资源丰富, 种活性成分。药理作用研究证实,其具有抗肿瘤、抗炎、抗病蕴藏量大,每年春天采收茎枝后,翌年仍生长茂盛。红毛五加与毒、抗辐射、保肝、调节心脑血管及机体免疫功能作用等“。7’。其它五