Windhorst, Johansson - Modern Techniques in Neuroscience Research.pdf

Contents
Chapter 1
Cytological Staining Methods
Robert W. Banks
Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Subprotocol 1: Fixation, Sectioning and Embedding . . . . . . . . . . . 2
Subprotocol 2: Ultrastructure . . . . . . . . . . . . . . . . . . . . . 9
Subprotocol 3: The Golgi Method . . . . . . . . . . . . . . . . . . . 13
Subprotocol 4: Single-Cell Methods. . . . . . . . . . . . . . . . . . . 17
References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Chapter 2
Application of Differential Display and Serial Analysis
of Gene Expression in the Nervous System
Erno Vreugdenhil, te de Jong and Nicole Datson
Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Subprotocol 1: Differential Display: Practical Approach. . . . . . . . . . 30
Subprotocol 2: Serial Analysis of Gene Expression (SAGE): Practical Approach39
Subprotocol 3: Digestion of cDNA with Anchoring Enzyme . . . . . . . . 43
Subprotocol 4: Binding to ic Beads. . . . . . . . . . . . . . . . 44
Subprotocol 5: Addition of Linkers . . . . . . . . . . . . . . . . . . . 45
Subprotocol 6: Tag Release by Digestion with Tagging Enzyme. . . . . . . 46
Subprotocol 7: Blunting Tags . . . . . . . . . . . . . . . . . . . . . 47
Subprotocol 8: Ligation to Ditags. . . . . . . . . . . . . . . . . . . . 47
Subprotocol 9: PCR Amplification of Ditags . . . . . . . . . . . . . . . 48
Subprotocol 10: Ditag Isolation . . . . . . . . . . . . . . . . . . . . 49
Subprotocol 11: Concatemerisation. . . . . . . . . . . . . . . . . . . 51
Subprotocol 12: Cloning Concatemers . . . . . . . . . . . . . . . . . 52
Subprotocol 13: Sequencing . . . . . . . . . . . . . . . . . . . . . . 53
References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Chapter 3
Methods Towards Detection of Protein Synthesis in Dendrites and Axons
Jan van Minnen and . van Kesteren
Introduction. . . . . . . . . . . . . . . . . .