2010全国生物材料大会论文集之 (228).pdf

Establishment and Identification of Cold-stressed
Normalized Full-Length cDNA Library of Ceratoides
lanata

Dang-Quan ZHANG*, Fu-Huan MING, Quan-Dong Ping JIANG
ZHU, Zhi-Dan SONG, Xin HAN School of Civil Engineering and Mechanic,
Biotechnology Core Facilities / Key Lab of Non-wood Lanzhou University,
Forest Products of State Forestry Administration, Lanzhou 730000, . China
Central South University of Forestry and Technology,
Changsha 410004, . China
* E-mail: NWFP_zhangdq@


Abstract—Ceratoides lanata is the native sand-fixing plant in of plant antifreeze activity involves the increasing
deserts of America Continent. As an even-green undershrub with accumulation of some free amino acids and augment of
extremely strong antifreeze capability, Ceratoides lanata had been unsaturation degree of fatty acids in membrane lipid [2-3].
considered as the winterfat and introduced into many countries However, researches about the relationship between plant
including China. To explore the mechanism of cold resistance antifreeze activity and some newly found substance by ic
and to clone genes directly involving the strong antifreeze engineering in recent years suggested that freeze-resistant
capability, a normalized cold-stressed full-length cDNA library plants are directly related to the in vivo expressed antifreeze
was constructed from