2010全国生物材料大会论文集之 (978).pdf

In Vitro Screening of Antioxidant Substances by
Fading Spectrophotometric
Qin Qin, Wenjian Shi*, Hualan Zhou, Yuanzhang Zhang
School of Environment and Architecture
University of Shanghai for Science and Technology
Shanghai 200093, People’s Republic of China

Abstract—A simple method for the determination of hydroxyl methods, spectrophotometry is superior in many aspects: the
radical was established to screen the antioxidant substances and equipment needed is inexpensive and easy to operate, the
evaluate their antioxidation ability. In this paper, effect of pH on selectivity and sensitivity of spectrofluorometry are high, and
absorption spectrum of dyes, the optimal experimental condition reproducibility is good.
of the fading reaction, the ics of degradation of dyes by Seven dyes test is a new test that qualitatively detect
Fenton oxidation, the relationship between the molecular hydroxyl radicals. The seven dyes includes Victoria Pure Blue
structure of dyes and reaction rate, effect of co-existing ions on
the reaction were investigated. Results indicate that the BO(VPB BO), Victoria Pure Blue B(VPB B), Victoria Pure
absorption spectrum of dyes do not shift and the position of Blue R(VPB R), Night Blue(NB), Rhodamine B(Rh B),
absorption peak keep stable when the pH value is higher than 3. Malachite Green(MG) and Methylene Blue(MB). A Fenton