2010全国生物材料大会论文集之 (981).pdf

Acute toxicity and mutagenicity of extract from
Eriobotrta japonica

Department of Chemistry Engineering Department of Chemistry Engineering
Huainan Union University Huainan Union University
Huainan,China Huainan,China
E-mail:humingbaohnuu@ E-mail: anhuidonghaili@


Abstract—To study the acute toxicity and the mutagenicity of The activating efficiency of S9 was verified using indirect
Eriobotrya japonica extract. The oral acute toxicity test in mice , mutagen 2-AF.
Ames test , micronucleus test in bone marrow and sperm
abnormality test were carried out. The reults shown that 2-Aminofluorene (2-AF), Cyclohosph amide were
tolerated dose of 20g/kg body weight in mice could be regarded purchased from Dayaofang Chemical and Medicine Co.(
as virtually non-toxic. The micronucleus rates and sperm Huainan, China).
abnormality rates in all doses were not significantly different
from the control group. No increase in the number of revertant B. Extraction of the plant materials
colonies was found in the Ames test with and without S9 Dried and powered leaves of Eriobotrta japonica was
acute toxicity and the mutagenicity of Eriobotrya
japonica extract were not observed in this study. extracted with 95% ethanol at room (20±2℃) for 24h and the
extract was concentrated under vacuum to produce a extract of
Keyword