2010全国生物材料大会论文集之 (983).pdf

HPLC Determination chlorogenic acid in Do-Zhong
(mia ulmoides) leaves by ultrasolic extraction

Hong Liu
Department of Chemistry Engineering Department of Chemistry Engineering
Huainan Union University Huainan Union University
Huainan,China Huainan,China
humingbaohnuu@ Jinxueyuan18888@


Abstract—In order to analysis of chlorogenic acid in Do- been an increasing interest in the use of ultrasound extraction
Zhong(mia ulmoides), ultrasolic extraction was used to extraction [12] .
prepare the sample. With yield of chlorogenic acid as index, The
effect of ethanol concentration, ultrasolic power. extraction time, The objective of this work was to establish a rapid and
and the ratio of solid to liquid were studied. The extracts were reliable routine analytical bining ultrasolic
then analysis by high-performance liquid chromatographic extraction and HPLC for determination of chlorogenic acid in
(HPLC) method. The results shown that the optimum extraction Do-Zhong leaves.
conditions were as follows: 70% ethanol(w/w) , extraction power
200W, extraction time 18 min, stock ratio 1:10(g:mL). For HPLC II. MATERIAL AND METHOD
analysis, the calibration plot was linear (R2=) in the
concentration range 200~1000μg/mL for chlorogenic acid,The A. material
method was precise with relative standard deviation for
chlorogenic acid ran