ELISA(酶联免疫吸附测定)实验报告.doc

ELISA(酶联免疫吸附测定)实验报告.docELISA
Lin Che ngyu Bio 04 2010030007
Experime nt Date: 2012-03-12 Submitt ing Date: 2012-03-21
Introduction
Background information
ELISA (Enzyme-linked Immunosorbent Assay) is a solid-phase assay for antibodies emplo ying liga nds labeled with en zymes which is widely used for immuno logical assays.
This technique can be applied to detect antigens or antibodies for qualitative or qua ntitative purpose. Si nee en zyme reacti ons are very well known amplificati on processes, the sig nal is gen erated by en zymes which are lin ked to the detect ion reage nts
1
in fixed proportions to allow accurate quantification.
Major principles
Prnd jtl
Induct ELISA
❷ 日locking butTer is added [□ bluJ^ trridlrnng MOteobindiig setei
Next n KJtiDlr primary ndeed
0 A ^L11 '^tle f ocorti/afy af>t)t>e)dy HfiPO I* tun a deed sHizih 运 and lo tn&
pm ary annbad^
Q TNFJ =fWi' No T113- Sflrtnn
aid is convened by Hf?=C :q a讯务JEEfcrm
Figure 2 Procedure of indirect ELISA
As show n in Figure 1 & 2, the gen eral procedure of in direct ELSIA is to: in cubate the
st
plate well with antigen, wash off unbounded antigen, incubate with 1 antibody, wash off unbounded 1 st antibody, incubate with labeled 2 nd antibody, wash off unbounded 2 nd antibody, incubate with enzyme substrate solution, and detect optical density or other in dex show ing en zyme activity.
Experiment Operation
Anti