麝香、冰片对全脑缺血再灌注大鼠脑组织氨基酸类神经递质的影响.pdf

·82· 《中药新药与临床药理》创刊20周年优秀论文集麝香、冰片对全脑缺血再灌注大鼠脑组织氨基酸类神经递质的影响刘亚敏,张赐安,徐秋英,彭胜权(广州中医药大学第一临床医学院,广州510405) 摘要:目的探讨芳香开窍药麝香、冰片对全脑缺血再灌注大鼠脑组织氨基酸类神经递质的影响。方法 Pulsinelli的4vo法建立大鼠全脑缺血再灌注模型,采用高效液相色谱法检测脑组织谷氨酸、天门冬氨酸、甘氨酸及^y一氨基丁酸。结果芳香开窍药可降低缺血区脑组织中的Asp,提高GABA、Gly的含量。结论芳香开窍药可降低脑缺血时的兴奋性神经递质Asp和升高抑制性神经递质GABA、Gly,以对抗兴奋性氨基酸的毒性, 从而保护脑缺血后继发神经元的损伤,这可能是其芳香开窍机理之一。关键词:麝香;冰片;脑缺血再灌注;神经递质;大鼠中图分类号:; 文献标志码:A 文章编号:1003—9783(2011)S一0082—03 Effect ofMo∞hm and Borneol on theContent ofAminoacid Neurotransmitter inBrain Tissue ofRats with Cere·-bral Ischemia and Reperfusion Injury LIU Yamin,ZHANG Ci'an,XU Qiuying,et a1.(Guangzhou University ofTraditional Chinese Medicine,Guangzhou 510405) Abstract: Objective Toinvestigate theeffectofresuscitation—inducing aromatic herbs such asMoschus andBomeol on aminoacid neurotransmitter content inbrain tissueof ratswith cerebral ischemia and reperfusion Rat model of cerebral ischemia and reperfusion injury was established by Pulsinelli 4vo content ofSin— tamicacid(Glu),asparticacid(Asp),glycine(Gly)and 1/-aminobutyrie acid(GABA)in braintissue were measured by high performance liquid chromatographic determination(HPLC).Results Resuscitation—inducing aromatic herbs Call decreaseAsp content andincrease GABA and Gly content inthebrain Resuscitation—indue- ing aromatic herbs can prevent brain tissue from ischemic lowering th