运用生物传感器检测mecA耐药基因.docx

运用生物传感器检测mecA耐药基因
Biotechnology Frontier
April 2022, Volume 4, Issue 1, -25 MecA Resistant Gene Detectio药的机制主要是由青霉素结合蛋白PBP2a介导的，SCCmec中的mecA基因是PBP2a的编码基因[6-8]，所以通过检测mecA基因中的一段特异性序列可以评估金黄色葡萄球菌对甲氧西林的耐药性[9-11]。
目前，细菌耐药性的检测方法主要分为常规表型检测（药敏试验）和耐药基因检测。常规药敏试验在国内实验室常规检测中比较常用，但需要至少2-3 天的培养时间，而且许多生长缓慢和不易培养的细菌，无法通过常规药敏试验检测其耐药性。以DNA探针和聚合酶链式反应为基础的实时荧光定量PCR技术可以从分子生物学水平检测细菌耐药。但是，常规分子生物学方法对每一个测试的抗菌药物，均需要设计相应的分
Biotechnology Frontier
April 2022, Volume 4, Issue 1, -25 MecA Resistant Gene Detection Based on Biosensor
Ting Wang, Zhang Zhang, Guoming Xie#
Key Laboratory of Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, 400016, China


#Email: ******@
Abstract
A novel electrochemical strategy was designed for the detection of methicillin-resistant staphylococcus epidemidis resistant gene
using methylene blue as signal tag. First, the hairpin probes are immobilized on the gold electrode, and the MCH are used to close non-specific binding sites on the surface of the electrode. Next, the immobilized hairpin probes recognized the targets to trigger primer extension reaction by target-induced conformational transition, leading to an ultrasensitive electrochemical bioe