腺花素对急性肺损伤模型小鼠的保护作用及其机制 司徒杰.pdf

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医药导报
目；苏木精-伊红（HE）染色观察肺组织病理学改变。
采用噻唑蓝（MTT）法检测不同浓度腺花素对
细胞生长的影响。AnnexinV-FITC/PI 双染法检测腺花
素对 RAW 细胞凋亡的影响。免疫印迹法（Westernblotting）检测 RAW 细胞和肺组织中 TLR4、
MyD88、p-NF-κB、NF-кB、Bcl-2、Bax 及β-Tubulin
的表达。 结果 腺花素显著降低 TNF-α、IL-1β、IL-6
和肺泡灌洗液中总蛋白浓度及巨噬细胞和中性粒细胞
的百分比，显著改善肺组织病理损伤；0～12μmol·L-1
腺花素对巨噬细胞 生长无明显抑制作用；腺
花素显著抑制脂多糖诱导的 RAW 细胞凋亡以及
TLR4、MyD88、p-NF-кB、Bax 的表达，促进 Bcl-2 的
表达；显著抑制肺组织中 TLR4、MyD88、p-NF-кB、
Bax 的表达，促进 Bcl-2 的表达。 结论 腺花素可
改善 ALI，其机制可能是抑制 TLR4/MyD88/NF-κB 炎
症通路并减少细胞凋亡。
关键词 腺花素；急性肺损伤；TLR4/MyD88/NF-κB

Effects of Adenanthin on Acute Lung Injury and
the Mechanic Analysis
SI Tujie1 ZENG Cheng2(1. Department of Pharmacy,
the First Affiliated Hospital of Guangzhou
University of Traditional Chinese Medicine,
Guangzhou 510405, China; 2. Center for Drug
Research and Development, Guangdong
Pharmaceutical University, Guangzhou 510006,China)
Abstract: Objective To investigate the
therapeutic effect and mechanism of
Adenanthin(Ade) on acute lung injury (ALI).
Methods Thirty male C57BL/6 mice were randomly
divided into 6 groups: normal group, model group
(lipopolysaccharide (LPS) group), positive drug
group (5 mg • kg-1 dexamethasone group) and
Adenanthin with different dose group (5, 10 and
20 mg • kg-1). After 6 hours of modeling,
therapeutic drugs were given. After 18 hours,
weight changes were recorded. The contents of
TNF-α, IL-1β and IL-6 in lung tissue homogenate
were determined by ELISA. The concentration of
total protein, the numbers of macrophages and
neutrophils were determined by BCA colorimetry
and Ray-Giamsa staining in alveolar lavage fluid,
respectively. The histopathological changes of
l