心痛贴对急性心肌梗死犬氧化应激、心肌代谢和超微结构的影响.doc

1 心痛贴对急性心肌梗死犬氧化应激、心肌代谢和超微结构的影响作者: 崔新明王秋静谷欣权曹霞路航刘芬吕文伟【摘要】目的观察心痛贴( XTT ) 对犬急性心肌梗死的保护作用机制。方法 30 只犬随机分成 5组: 假手术组、模型组、 XTT 小剂量组、大剂量组、阳性药对照组。结扎犬冠状动脉左前降支( LAD ) 复制急性心肌梗死模型,将 XT T 贴于犬左前胸近腋窝部位,测定给药后 6h 血清超氧化物歧化酶( SOD ) 、脂质过氧化物(LPO) 、游离脂肪酸( FFA )、氧化氮( NO ) 含量变化, 透射电镜观察心肌细胞超微结构改变。结果 XTT 能降低血清 FFA 及 LPO 含量,提高 NO 含量和 SOD 活性( 、 ) ,可改善心肌梗死后心肌细胞超微结构。结论 XTT 可改善心肌细胞超微结构,对缺血心肌具有明显保护作用, 与其增强抗氧化酶活性, 减少脂质过氧化反应,纠正心肌 FFA 代谢紊乱,提高 NO 水平有关。【关键词】心痛贴;心肌梗死;超微结构;氧化应激; 心肌/ 代谢 2 【 Abstract 】 Objective To observe the protective mechanism of Xintong Tie (XTT )on acute myocardial infarction(AMI) in dogs. Methods Thirty dogs were divided into sham operation, model, low 拟 dose XTT ( g), high 拟 dose XTT( g) and positive control groups at random. An AMI model was set up by ligating left anterior descending branch(LAD) in anesthetic chest 拟 opened dog. The patch was pot on the left chest near the armpit of every dog respectively. The content of SOD, LOP, FFA and NO were determined after 6 h. The changes of myocardial ultrastructural were observed. Results XTT reduced the ultrastructural injuries in AMI dogs. The contents of FFA and LOP were decreased , the activities of NO and SOD were increased (P< , ). Conclusions XTTi can improve the myocardial ultrastructure of ischemic myocardium with a significant protective effect , enhance the activity of antioxidant enzymes , reduce lipid peroxidation