锌对铅中毒大鼠海马nNOS神经元的影响.doc

1 锌对铅中毒大鼠海马 nNOS 神经元的影响作者:熊伟,赵英,万炜,陈锋,李素云【摘要】目的: 探讨锌对铅暴露大鼠海马 nNOS 神经元的保护作用。方法:4 周龄健康 Wistar 雄性大鼠 48只, 分为对照组、铅中毒组、葡萄糖酸锌预防组和葡萄糖酸锌治疗组,每组 12 只。对照组用去离子水灌胃 40d ;铅中毒组大鼠用 15 mg/(kg · bw · d) 醋酸铅灌胃 30d ,去离子水灌胃 10d; 锌预防组用 30 mg/(kg · bw · d) 葡萄糖酸锌和 15 mg/(kg · bw · d) 醋酸铅联合灌胃 30d, 后用去离子水灌胃 10d; 锌治疗组用 15 mg/(kg · bw · d) 醋酸铅灌胃 30d, 后用 30 mg/(kg · bw · d) 葡萄糖酸锌灌胃 10d; 对照组、铅中毒组、锌预防组和锌治疗组在第 40 天处死大鼠取海马,分别进行 NOS 活性测定; β拟 NADPH 组织化学方法观察海马 nNOS 数量的变化。结果: 锌预防组和锌治疗组大鼠海马 NOS 活性高于铅中毒组而低于对照组;锌预防组大鼠海马 NOS 活性高于锌治疗组; 在 CA1 区和齿状回, 除锌预防组外, 其它各组 nNOS 阳性神经元数量明显低于对照组, 反应强度变弱; 锌预防组和锌治疗组 nNOS 阳性神经元数量高于铅中毒组; 锌预防组大鼠海马 nNOS 阳性神经元数量高于锌治疗组。结论:补锌对铅中毒 2 大鼠海马 nNOS 神经元的活性和数量都有提高, 预防性补锌的效果更好。【关键词】锌;铅暴露;海马; nNOS 神经元 Influence of Zinc on lead poisoning Hippocarnpus nNOS Neuron Rats Abstract : Objective To approach protective effect of zinc on lead poisoning hippocampus nNOS neuron of 4 weeks old male wistar rats were divided into 4 groups randomly: blank group 、 lead poison group 、 precaution group with Zinc Gluconate and therapy group with Zinc Gluconate. The blank group , administrated intragastrically with the dEionized water for 40 days ; The Lead poisoned group , administered intragastrically with 15 mg/(kg · bw · d) lead acetate for 30 days and with the dEIonized water for next 10 days; The precaution group , administered intragastrically with 15 mg/(kg · bw · d) lead acetate and 30 mg/(kg · bw · d) Zinc Gluconate for 30 days and with the deionized water for next 10 days ;The therapy groups , administered intragastrically with 15 mg/(kg · bw · d) lead acetate for 30 days , followed by treatment with 30 mg/(kg · bw · d) Zinc 3 Gluconate for next 10 days; On 40th days , the vats wero killed and the activity of NOS of hippocampus was measured. The nNOS positive neuron amount was measured byβ拟 NADPH histochemistry. Results The activity of NOS of hippocampus of the precaution group and the therapy group was higher than the lead poisoned group and was lower than the blank group; The activity of N