阴沟肠杆菌对乐果降解作用因素的研究.doc

阴沟肠杆菌对乐果降解作用因素的研究
官雪芳2 林抗美1 马丽娜1 鲁国东3 王宗华3 刘波1*
(1 福建省农业科学院生物技术研究所,福建福州350003,2福建省农科院农业工程技术研究所,福建福州350003 , 3 福建农林大学生命科学学院,福建福州 350002)
摘要: 研究各因素对阴沟肠杆菌降解乐果效能的影响,结果为:菌降解乐果的最佳温度为35℃、、装液量50ml/250ml、摇床振数180r /min、C源为果糖、N源为酵母浸膏、、底物浓度为200mg/L,%、%、%、%、%、%、%、40%,采用L18(2*37)正交法及DPS数理统计得出Cu2+,Fe2+对细菌降解乐果有较强的促进作用。正交试验筛选菌株降解乐果的最佳发酵条件组合为:碳源:蔗糖;离子:CuCl2:(10 Mmol/L);乐果浓度:100mg/L;培养时间36h;培养温度30℃;pH值8;氮源:酵母3g/L,%;其中离子对菌降解乐果的影响最为显著。
关键词:阴沟肠杆菌乐果降解气相色谱液相色谱
Study on influencing factors of the degradation to Dimethoate of
GUAN Xuefang2,LIN Kangmei1, MA Lina1,LU Guodong3, WANG Zonghua3,LIU Bo1*
(1Biotechnology Institute,Fujian Academy of Agricultural Sciences,Fuzhou,350003, China),(2 Institute of AgriculturalEngineering Technology, Fujian Academy of Agricultural Sciences,Fuzhou,350003, China),(3 Life Science College, Fujian Agriculture and Forestry University, Fuzhou 350002, China)
Abstrat:The effective factors of the degradation to Dimethoate of were studied. The result showed that the optimum temperature, initial pH,volume,rotate speed ,carbon source,nitrogen source, inoculum size and the density of dimethoate for the strain to degrading were 35℃, , 50ml/250ml, 180r /min, fructose,yeast extract,OD265: with the degrading rates of %, %,%,%,%,%,