VEGF在胃癌患者血清与肿瘤组织中的表达及其意义 PPT课件.ppt

VEGF在胃癌患者血清及肿瘤组织中的表达及其意义
[摘要]  目的   研究血管内皮细胞生长因子(VEGF)在胃癌患者血清及肿瘤组织中的表达情况,探讨其临床意义。方法  应用ELISA法对60例非肿瘤疾病手术患者和60例胃癌患者术前、术后血清VEGF水平进行定量测定,并应用免疫组化法测定肿瘤组织的VEGF。结果  胃癌患者术前血清VEGF高于对照组(P>)。两组术后较术前升高,肿瘤组术后较术前升高(P<)。肿瘤组术前血清VEGF水平、肿瘤组织中的VEGF与浆膜受累、淋巴结转移及临床病理分期有关。肿瘤组术后随访30~36个月中出现复发转移和死亡的患者,术前VEGF表达较其他患者升高(P<)。结论  血清VEGF水平及肿瘤组织中的VEGF与胃癌的浸润、转移有关,检测血清VEGF及肿瘤组织中的VEGF对胃癌浸润、转移程度和预后的判断有重要意义。
[关键词]  胃肿瘤;微转移;血管内皮生长因子        Expression and significance of VEGF in serum and tumor tissue in gastric cancer        ZHOU Hai-yin,LI Jian,QIAO Shi-ming,et of General Surgery,The Central Hospital of Yangpu District,Shanghai 200090,China
[Abstract]  Objective  To evaluate the clinical significance of vascular endothelial growth factor (VEGF) expression in serum and tumor tissue in gastric cancer   The pre-operational and post-operational levels of serum VEGF were measured by ELISA kit in 60 cases of gastric cancer and 60 cases of non-carcinoma as the control group,the expression of tumor tissue VEGF was measured by immunohistochemical   The pre-operational level of serum VEGF in gastric cancer was higher than that in the control group(P>).
The post-operational level of serum VEGF was increased in both groups,especially in the tumor group(P<).The expression of VEGF in serum and tumor tissue was positively correlated with tumor invasion depth,lymph-node metastasis and clinical pathological follow up of 30~36 months reveals the pre-operational level of serum VEGF of tumor recurrence and death in patients was higher than the others in the tumor group(P<).
Conclusion  The expression of serum VEGF and tumor tissue VEGF is closely correlated with invasion and metastasis of gastric VEGF and tumor tissue VEGF assay may be used to pre-operatively predict invasion and metastasis and used to evaluate prognosis.
[Key words]  stomach neoplasm;micrometastasis;vascular endothelial growth factor        肿瘤的增殖和转移是血管新生依赖