体外骨形态发生蛋白4抑制乳腺癌细胞的增殖和迁移.pdf

CHINESE JOURNAL OF ANATOMY 2021 解剖学杂志 2021 年第 44 卷第 2 期

of breast cancer cells in vitro*
Li Haiyun， Yang Dongguang， Hong Xiuli， Li Jinbo， Wang Hui
（Department of General Surgery， Zhuozhou City Hospital Zhuozhou 072750， China）
Abstract  Objective ： To study the effect of bone morphogenetic protein 4（ BMP4） on the proliferation， migration
and invasion of breast cancer cell line MCF-7 in vitro. Methods ： MCF-7 cells were infected with lentivirus Lv-BMP4
and lentivirus Lv-shBMP4 to construct MCF-7/Lv-BMP4 overexpression group and MCF-7/Lv-shBMP4 interference
group. There were 4 groups in this experiment: Blank group， Lv-NC group， Lv-BMP4 group and Lv-shBMP4 group.
RT-PCR and ELISA were used to detect BMP4 overexpression and interference in MCF-7 cells. CCk-8 assay was
used to detect the proliferation of MCF-7 cells. Scratch healing and Transwell were used to detect the migration and
invasion ability of MCF-7 cells. Results ： RT-PCR and ELISA showed that the BMP4 over-expressed breast cancer cell
lines MCF-7/Lv-BM