激光粒度仪测定阿霉素纳米颗粒的粒径及粒径分布.doc

激光粒度仪测定阿霉素纳米颗粒的粒径及粒径分布
吕凤娇1 陈晓耕2 林华香1 许小平1#
(1福州大学化学化工学院福建福州 350002;2 福建省省立医院 350000)
摘要:本文以阿霉素为模型药物,N-乙烯基吡咯烷酮为单体,通过反相乳液聚合法制备了聚乙烯吡咯烷酮为载体的阿霉素抗癌纳米颗粒,并用激光粒度仪测量了纳米颗粒的粒径大小和粒径分布,同时研究了乳化剂浓度、引发剂浓度、体系水量、反应温度对纳米颗粒尺寸的影响。
关键词:阿霉素;纳米颗粒;激光粒度仪;粒径;粒径分布
中图分类号:TQ46 文献标识码:A
Determination of Doxorubicin-loaded nanoparticles size and
size distribution with laser particle size instrument
LV feng-jiao1 CHEN xiao-geng2 LIN hua-xiang1 XU xiao-ping1#
( of Chemistry and Chemical Engineering, Fuzhou University, Fujian Fuzhou 350002;
2. Fujian Provincial Hospital Fuzhou 350000)
Abstract:Doxorubicin-loaded nanoparticles, using doxorubicin as model drug and polyvinylpyrrolidone as matrix material, were prepared by inverse emulsion polymerization in this paper. The mean particle size and size distribution were measured by laser light scattering technique. The effect factors, such as the emulsifier, the initiator,the water and the temperature
,on the size and the size distribution of Doxorubicin-loaded nanoparticles were investigated. Keywords: doxorubicin; nanoparticle; laser light scattering technique; size; size distribution
纳米药物是国际上刚兴起的一种新型给药系统[1],它以高分子材料为载体, 药物通过雾化法[2]、化学沉积法[3]、溶剂蒸发法[4]、薄膜-超声分散法[5]和乳液聚合法[6]等包覆在高分子材料中。纳米药物制备的关键是控制粒子的大小和获得较窄且均匀的粒度分布,减少粒子团聚现象,保证用药有效、安全和稳定。目前,测量纳米颗粒尺寸的仪器主要有透射电镜、环境扫描电镜、X射线衍射仪、激光粒度仪等。透射电镜和环境扫描电镜只能给出局部粒径的大小;X射线衍射仪得到的只是单个晶包的尺度。激光粒度仪以激光为相干光源,通过探测粒子