Allyl isothiocyanate ameliorates insulin resistance through the regulation of mitochondrial function.pdf.pdf

Available online at
ScienceDirect
Journal of Nutritional Biochemistry 25 (2014) 1026–1034
Allyl isothiocyanate ameliorates insulin resistance through the regulation of
mitochondrial function
Jiyun Ahna,b, Hyunjung Leea, Sung Won Ima, Chang Hwa Junga,b, Tae Youl Haa,b,⁎
aMetabolism and Nutrition Research Group, Korea Food Research Institute, Seoungnam, Korea
bDivision of Food Biotechnology, Korea University of Science and Technology, Daejeon, Korea
Received 3 March 2014; received in revised form 21 April 2014; accepted 7 May 2014
Abstract
Mitochondrial dysfunction is associated with the pathophysiology of insulin resistance. Allylisothiocyanate (AITC) is found in many cruciferous vegetables and has
been reported to possess anticancer activity. However, the effect of AITC on insulin resistance and mitochondrial function has not yet been investigated. Here, we show
that AITC increased glucose uptake in insulin-resistant C2C12 myotubes and augmented glucose transporter 4 (GLUT4) translocation in L6-GLUT4myc cells. AITC
recovered the impaired insulin signaling evoked by free fatty acid exposure and increased mitochondrial membrane potential and mitochondrial DNA content. AITC also
elevated the rate of oxygen consumption in C2C12 cells. Furthermore, mice that were fed a high-fat diet with AITC for 10 weeks had reduced diet-induced obesity and
hepatic steatosi