Cell wall-associated Mycobacterium tuberculosis rRv3083 protein stimulates macrophages through toll-like receptor-2 (TLR2).pdf.pdf

International Journal of Mycobacteriology 4 (2015) 176
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Cell wall-associated Mycobacterium tuberculosis
rRv3083 protein stimulates macrophages through
toll-like receptor-2 (TLR2)
Iti Saraav, Swati Singh, Kirti Pandey, Ekta Vishnoi, Monika Sharma, Sadhna Sharma *
. Kothari Centre for Research and Innovation in Science Education, Department of Zoology, Miranda House, University of Delhi, Delhi, India
ARTICLE INFO
Article history: Aims and objectives: Characterization of proteins associated with the mycobacterial cell
Received 21 June 2014 wall is critical to understanding bacterial survival and immune modulation in the host.
Accepted 25 June 2014 A variety of mycobacterial products are able to recognize and activate mammalian toll-like
Available online 22 July 2014 receptors (TLRs) mediating the secretion of antibacterial effector molecules. Mycobacterium
tuberculosis MymA Rv3083 protein is a cell wall-associated protein which is up-regulated
Keywords: upon infection of macrophages. The objective of the present study is to understand the role
M. tuberculosis of Rv3083 protein as a TLR agonist and its contribution in activating macrophages.
Rv3083 Methods: The MymA (Rv3083) gene was cloned and expressed. The purified kDa -
MymA binant protein was used to stimulate phorbol my