Molecular cloning, expression profiles and promoter analysis of insulin-like growth factor binding protein-4 (IGFBP-4) in Japanese flounder (Paralichthys olivaceus).pdf.pdf

Comparative Biochemistry and Physiology, Part B 175 (2014) 41–52
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Comparative Biochemistry and Physiology, Part B
journal homepage: ate/cbpb
Molecular cloning, expression profiles and promoter analysis of
insulin-like growth factor binding protein-4 (IGFBP-4) in Japanese
flounder (Paralichthys olivaceus)
Jing Wang, Jinning Gao, Wenji Wang, Liman Ma, Mengmeng Liu, Haiyang Yu, Zhigang Wang, Xubo Wang,
Jie Qi, Quanqi Zhang ⁎
College of Marine Life Sciences, Ocean University of China, Key Laboratory of Marine ics and Breeding, Ministry of Education, #5 Yushan Road, Qingdao 266003, China
article info abstract
Article history: We cloned and characterized cDNA sequence of insulin-like growth factor binding protein-4 (IGFBP-4) from
Received 30 December 2013 Japanese flounder (Paralichthys olivaceus). The 1493 bp full-length cDNA sequence contained an open reading
Received in revised form 24 May 2014 frame (ORF) of 780 bp, which encoded a protein of 259 amino acids. The deduced amino acid sequences included
Accepted 20 June 2014
a putative signal peptide of 28 amino acid residues resulting in a mature protein of 231 amino acids. Twenty cys-
Available online 28 June 2014
teine residues and two conserved IGFBPs motif (XXC and CWCV) were found in the N- and C-terminal do-
main. In the over 13 kbp genomic sequence, four exons, three introns, and 5′-/3′-flanking sequences were
Keywords:
fifl
Paralichthys olivaceus identi ed. Sequence alignment and ic analysis showed that Japanese ounder IGFBP-4 was indeed
Insulin-like growth factor binding protein-4 the ortholog of the human IGFBP-4 gene and shared high identities with other teleost IGFBP-4 genes. The promoter
cDNA cloning region was also analyzed and several potential transcription factor (TF) binding sites were determined which may
mRNA expression modulate the IGFBP-4 expression. Quantitative real-time PCR analysis revealed that IGFBP-4 mRNA was observed
Promote