G-protein coupled receptor 6 deficiency alters striatal dopamine and cAMP concentrations and reduces dyskinesia in a mouse model of Parkinson's disease.pdf.pdf

Experimental Neurology 257 (2014) 1–9
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Experimental Neurology
journal homepage: ate/yexnr
Regular Article
G-protein coupled receptor 6 deficiency alters striatal dopamine and
cAMP concentrations and reduces dyskinesia in a mouse model of
Parkinson's disease
Patrick Oeckl 1, Bastian Hengerer, Boris Ferger ⁎
CNS Diseases Research, Boehringer Ingelheim Pharma GmbH & Co. KG, Birkendorfer Str. 65, 88397 Biberach an der Riss, Germany
article info abstract
Article history: The orphan G-protein coupled receptor 6 (GPR6) is a constitutively active receptor which is positively coupled to
Received 14 February 2014 the formation of cyclic adenosine-3′,5′-monophosphate (cAMP). GPR6 is predominantly expressed in
Revised 7 April 2014 striatopallidal neurons. Here, we investigated neurochemical and behavioural effects of Gpr6 deficiency in mice.
Accepted 10 April 2014
Gpr6 depletion decreased in vivo cAMP tissue concentrations (20%) in the striatum. An increase of striatal tissue
Available online 18 April 2014
dopamine concentrations (10%) was found in Gpr6−/− mice, whereas basal extracellular dopamine levels were
+/+
Keywords: not pared with Gpr6 mice, as shown by in vivo microdialysis. Western blot analyses revealed
GPR6 no alteration in the expression and subcellular localisation of the dopamine D2 receptor in the striatum of