Riboflavin (vitamin B-2) reduces hepatocellular injury following liver ischaemia and reperfusion in mice.pdf.pdf

Food and Chemical Toxicology 67 (2014) 65–71
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Food and Chemical Toxicology
journal homepage: ate/foodchemtox
Riboflavin (vitamin B-2) reduces hepatocellular injury following liver
ischaemia and reperfusion in mice
Sheila Cristina Sanches a, Leandra Naira Z. Ramalho a, Mariana Mendes-Braz a, Vânia Aparecida Terra b,
⇑
Rubens hini b, Marlei Josiele Augusto a, Fernando Silva Ramalho a,
a Department of Pathology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil
b Department of Pathological Sciences, State University of Londrina, Londrina, PR, Brazil
article info abstract
Article history: Riboflavin has been shown to exhibit anti-inflammatory and antioxidant properties in the settings of
Received 9 October 2013 experimental sepsis and ischaemia/reperfusion (I/R) injury. We investigated the effect of riboflavin on
Accepted 11 February 2014 normothermic liver I/R injury. Mice were submitted to 60 min of ischaemia plus saline or riboflavin treat-
Available online 18 February 2014
ment (30 lmoles/kg BW) followed by 6 h of reperfusion. Hepatocellular injury was evaluated by amino-
transferase levels, reduced glutathione (GSH) content and the histological damage score. Hepatic
Keywords: neutrophil accumulation was assessed using the naphthol method and by measuring myeloperoxidase
Riboflav