武汉商业项目考察报告.ppt

免疫因素与男性不育的相关性
【Abstract】 AIM: To examine the relationship bet antibody (AsAb), anticardiolipin antibody (ACA) and antitoxoplasma antibody in the sera of inferlile of males, and other indexes of semen and to study the correlation and mechanism of AsAb, ACA, antitoxoplasma antibody and infertility. METHODS: AsAb, ACA and antitoxoplasma antibody in sera from 286 cases of male infertile patients and 198 normal persons easured elinked immunosorbent assay (ELISA) and other indexes of semen a antibody (%) in the sera of infertile patients al controls (%, % and %, respectively, P<). The change of AsAb level could cause a remarkable decrease of sperm activity and a significant positive correlation ale infertility and may lead to a remarkable decrease of sperm activity, but it does not significantly affect sperm density and liquidization.
【Keyale; antisperm antibody; antibodies, anticardiolipin; toxoplasmosis; enzymelinked immunosorbent assay; semen
【摘要】目的: 观察不育男性血清抗精子抗体(AsAb),抗心磷脂抗体(ACA),抗弓形虫抗体与精液各指标之间的关系,揭示免疫因素与不育症的相关性及致病机制. 方法: 用ELISA(Enzymelinked immunosorbent assay)法测定286例男性不育患者及198例正常生育者血清的AsAb, ACA,抗弓形虫抗体并常规检测精液的各项指标. 结果: 不育者血清中的AsAb, ACA, 抗弓形虫抗体阳性率(%, %, %)明显高于正常生育组(%, %, %),P<;不育患者血清AsAb水平变化能导致精子的活动力明显下降,呈显著性正相关, P<. 结论: AsAb与男性不育有密切关系,可引起精子活率、活力明显下降,但对精子密度及液化无明显影响.
【关键词】不育,男(雄)性;抗精子抗体;抗体,抗心脂;弓形虫病; 酶联免疫吸附测定;精液
0引言
近年来的研究表明,男性不育症的病因十分复杂,除输精管阻塞、腮腺炎病毒感染等明确病因外,相当一部分患者找不出原因[1]. 目前认为职业、环境因素可致男性不育,还有资料报道白细胞精子症导致NO浓度升高[2],过量NO又与无精症有密切相关[1]. 特别免疫因素是世界各国学者所关注研究的问题,,揭示抗精子抗体(AsAb),抗心磷脂抗体(ACA),抗弓形虫抗体与精液质量的关系,我们测定了286例男性不育患者及198例正常已生育者血清中的AsAb,ACA,抗弓形虫抗体并对其与精液各指标的关系进行了深入的分析.
1对象和方法

男性不育患者286例,均结婚2 a以上,***正常,年龄24~48()岁,女方经妇产科检查,,年龄26~35()岁,两组间年龄无显著性差异(P&lt;).

①标本: 检测对象抽取空腹静脉血2 mL,离心后取上层血清,-20℃贮存待测. 被测者均禁欲3~5 d后,在实验室用手***法留取精液于清洁的10 mL量杯中,液化后进行精液常规分析. ②试剂: ELISA法检测AsAb,ACA及抗弓形虫抗体,试剂盒均由华美生物工程公司生产. ③测定方法: 按WHO标准[3]对精液常规进行分析,如精液的体积、