HPLC法测定疗肺宁片中穿心莲内酯和脱水穿心莲内酯的含量.doc

HPLC法测定疗肺宁片中穿心莲内酯和脱水穿心莲内酯的含量
王保琳孙晓
(连云港市第一人民医院江苏连云港 222002)
【摘要】目的:建立高效液相色谱法测定疗肺宁片中穿心莲内酯和脱水穿心莲内酯含量的方法。方法:色谱柱:Ultimate XB-C18柱(5 ?m,×150 mm); ml/min;柱温:30℃;流动相:甲醇-水(52:48);用检测波长切换法;进样量:10 ?l。结果:~ ?g/ml时线性关系良好,r=,%;%(n=6);~ ?g/ml时线性关系良好,r=,%;%(n=6)。结论:该方法简单、准确,并且同时测定该制剂中两种有效成分的含量,可作为疗肺宁片的质量控制。
【关键词】高效液相色谱法;疗肺宁片;穿心莲内酯;脱水穿心莲内酯
【中图分类号】 【文献标识码】A 【文章编号】2095-1752(2015)17-0027-03
HPLC method for determination of cure lung ning in andrographis paniculta lactone content of lactone and dehydrations Wang Baolin Sun Xiao. The First People's Hospital of Lianyungang City, Jiangsu Province, Lianyungang 222002, China
【Abstract】Objectiv Establish a high performance liquid chromatography determination of cure pulmonary ning in paniculta lactone and dehydration of the content of method. Methods Chromatographic column: the Ultimate XB - C18 column (including 5 m, * 150 mm). The flow rate of ml/min; Column temperature: 30 ℃; Mobile phase: methanol-water (52:48); By detection wavelength switching method; Sample quantity: 10 (including l. Results Paniculta lactone in concentration of ~ (including g/ml good linear relation, r =
, the average recovery was %. The RSD was % (n = 6); Dehydration paniculta lactone in concentration of ~ (i